Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as fusion protein with glutathione S-transferase | Geobacillus virus E2 |
expression in Escherichia coli BL21 (DE3) as a fusion protein with glutathione S-transferase (GST) | Geobacillus virus E2 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
recombinant GVE2-TS protein, SDS-PAGE | Geobacillus virus E2 |
31000 | - |
x * 31000, SDS-PAGE and calculated | Geobacillus virus E2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Geobacillus virus E2 | thymidylate synthase is essential for de novo synthesis of dTMP and is a key enzyme involved in DNA synthesis and transcriptional regulation of organisms | 7,8-dihydrofolate + dTMP | - |
? | |
additional information | Geobacillus virus E2 | recombinant protein is able to bind its own RNA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus virus E2 | - |
GVE2, deep-sea thermophilic bacteriophage | - |
Geobacillus virus E2 | A6M9A2 | - |
- |
Purification (Comment) | Organism |
---|---|
GST-GVE2 TS fusion protein is purified by glutathione-Sepharose beads | Geobacillus virus E2 |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | expressed early after Geobacillus virus E2 infection | Geobacillus virus E2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Geobacillus virus E2 | 7,8-dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | thymidylate synthase is essential for de novo synthesis of dTMP and is a key enzyme involved in DNA synthesis and transcriptional regulation of organisms | Geobacillus virus E2 | 7,8-dihydrofolate + dTMP | - |
? | |
additional information | recombinant protein is able to bind its own RNA | Geobacillus virus E2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE and calculated | Geobacillus virus E2 |
Synonyms | Comment | Organism |
---|---|---|
Thy1 | - |
Geobacillus virus E2 |
ThyA | - |
Geobacillus virus E2 |