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Literature summary for 2.1.1.45 extracted from

  • Huang, X.; Gibson, L.M.; Bell, B.J.; Lovelace, L.L.; Pena, M.M.; Berger, F.G.; Berger, S.H.; Lebioda, L.
    Replacement of Val3 in human thymidylate synthase affects its kinetic properties and intracellular stability (2010), Biochemistry, 49, 2475-2482.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain TX61- cells and in the RJK88.13 cell line (an enzyme-deficient derivative of V79 Chinese hamster lung cells) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
V3L, V3F and V3Y mutant enzymes, hanging drop vapor diffusion method, using 100 mM Tris-base, pH 8.5, 20 mM 2-mercaptoethanol, 32-40% (w/v) ammonium sulfate and 16-24% (w/v) polyethylene glycol 4000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
V3A the mutant enzyme has an intracellular half-life of approximately 6 h after treatment with cycloheximide Homo sapiens
V3F the mutant enzyme has an intracellular half-life of approximately 3.5 h after treatment with cycloheximide Homo sapiens
V3L the mutant enzyme has an intracellular half-life of approximately 2.5 h after treatment with cycloheximide Homo sapiens
V3T the mutant enzyme has an intracellular half-life of approximately 24 h after treatment with cycloheximide Homo sapiens
V3Y the mutant enzyme has an intracellular half-life of approximately 2.5 h after treatment with cycloheximide Homo sapiens

General Stability

General Stability Organism
the wild type enzyme has an intracellular half-life of approximately 6 h after treatment with cycloheximide Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
dUMP apparent value, wild type enzyme, pH not specified in the publication, at 37°C Homo sapiens
0.0044
-
dUMP apparent value, mutant enzyme V3Y, pH not specified in the publication, at 37°C Homo sapiens
0.011
-
dUMP apparent value, mutant enzyme V3T, pH not specified in the publication, at 37°C Homo sapiens
0.022
-
dUMP apparent value, mutant enzyme V3A, pH not specified in the publication, at 37°C Homo sapiens
0.109
-
dUMP apparent value, mutant enzyme V3L, pH not specified in the publication, at 37°C Homo sapiens
0.134
-
dUMP apparent value, mutant enzyme V3F, pH not specified in the publication, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP
-
Homo sapiens dihydrofolate + dTMP
-
?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Homo sapiens