Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 0.001 50% inhibition | Cicer arietinum | |
Ba2+ | 1 mM, 25% inhibition | Cicer arietinum | |
Cd2+ | 1 mM, complete inhibition | Cicer arietinum | |
Cu2+ | 1 mM, complete inhibition | Cicer arietinum | |
Fe2+ | 1 mM, 78% inhibition | Cicer arietinum | |
Mn2+ | 1 mM, 43% inhibition | Cicer arietinum | |
S-adenosyl-L-homocysteine | - |
Cicer arietinum | |
Zn2+ | 1 mM, complete inhibition | Cicer arietinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
7,4'-dihydroxyisoflavone | pH 8.8, 30°C | Cicer arietinum | |
0.16 | - |
S-adenosyl-L-methionine | pH 8.8, 30°C | Cicer arietinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | does not require Mg2+ or other divalent cations | Cicer arietinum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Cicer arietinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | Cicer arietinum | i.e. daidzein. Methylation is the last step in the biosynthesis of 4-methoxyisoflavones | S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone | - |
? | |
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | Cicer arietinum | i.e. daidzein. Methylation is the last step in the biosynthesis of 4'-methoxyisoflavones | S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cicer arietinum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cicer arietinum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Cicer arietinum | - |
seedling | - |
Cicer arietinum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no methylation of: p-hydroxycinnamic acid, p-hydroxybenzoic acid, 3,4-dihydroxycinnamic acid, naringenin, (5,7,4'-trihydroxyflavanone), apigenin (5,7,4'-trihydroxyflavone), luteolin (5,7,3',4'-tetrahydroxyflavone), kaempferol (3,5,7,4'-tetrahydroxyflavone), wedelolactone (5,3',4'-trihydroxy-7-methoxycoumestone) | Cicer arietinum | ? | - |
? | |
S-adenosyl-L-methionine + 5,7,4'trihydroxyisoflavone | - |
Cicer arietinum | S-adenosyl-L-homocysteine + 5,7-dihydroxy-4'-O-methylisoflavone | - |
? | |
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | i.e. daidzein. Methylation is the last step in the biosynthesis of 4-methoxyisoflavones | Cicer arietinum | S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone | - |
? | |
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | i.e. daidzein. Methylation is the last step in the biosynthesis of 4'-methoxyisoflavones | Cicer arietinum | S-adenosyl-L-homocysteine + 7-hydroxy-4'-O-methylisoflavone | - |
? | |
S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone | i.e. daidzein. The reaction follows an ordered BiBi mechanism with S-adenosylmethionine and S-adenosylhomocysteine as leading reaction partners | Cicer arietinum | S-adenosyl-L-homocysteine + 7-hydroxy-4'-methoxylisoflavone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S-adenosylmethionine:isoflavone 4'-O-methyltransferase | - |
Cicer arietinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
in Tris-HCl buffer | Cicer arietinum |
9 | - |
in glycine-NaOH buffer | Cicer arietinum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | pH 7.5: about 40% of maximal activity (Tris-HCl buffer), pH 10.0: about 25% of maximal activity (glycine-NaOH buffer) | Cicer arietinum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
S-adenosyl-L-homocysteine | pH 8.8, 30°C, versus S-adenosyl-L-methionine | Cicer arietinum | |
0.3 | - |
S-adenosyl-L-homocysteine | pH 8.8, 30°C, versus 7,4'-dihydroxyisoflavone | Cicer arietinum |