Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.5 extracted from

  • Skiba, W.E.; Taylor, M.P.; Wells, M.S.; Mangum, J.H.; Awad, W.M.
    Human hepatic methionine biosynthesis. Purification and characterization of betaine:homocysteine S-methyltransferase (1982), J. Biol. Chem., 257, 14944-14948.
    View publication on PubMed

General Stability

General Stability Organism
divalent cations and 2-mercaptoethanol do not stabilize during purification Homo sapiens
substrates stabilize during purification Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,3-Dimethylbutyrate competitive to betaine Homo sapiens
Isovalerate competitive to betaine Homo sapiens
additional information not inhibitory: methionine or ethionine; product inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
betaine
-
Homo sapiens
0.12
-
L-homocysteine
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition Homo sapiens
45000
-
6 * 45000, SDS-PAGE Homo sapiens
270000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
two forms of the enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, many months in 50% v/v glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N,N-trimethylglycine + L-homocysteine
-
Homo sapiens N,N-dimethylglycine + L-methionine
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 45000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
significant heat stability Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
above, 4 h stable at 37°C Homo sapiens