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Literature summary for 2.1.1.5 extracted from

  • Li, F.; Feng, Q.; Lee, C.; Wang, S.; Pelleymounter, L.L.; Moon, I.; Eckloff, B.W.; Wieben, E.D.; Schaid, D.J.; Yee, V.; Weinshilboum, R.M.
    Human betaine-homocysteine methyltransferase (BHMT) and BHMT2: common gene sequence variation and functional characterization (2008), Mol. Genet. Metab., 94, 326-335.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in COS-1 cells and in Escherichia coli Homo sapiens
impossible to express BHMT2 in mammalian cells, protein aggregates after bacterial expression. BHMT2 is rapidly degraded in a rabbit reticulocyte lysate, but it can be stabilized by cotransfection of COS-1 cells with BHMT and, after cotransfection, it coprecipitates with BHMT Homo sapiens

Protein Variants

Protein Variants Comment Organism
A66V nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens
Arg16Cys nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0139 (betaine), 0.0076 (L-homocysteine) Homo sapiens
Arg239Gln nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.012 (betaine), 0.0158 (L-homocysteine) Homo sapiens
Gly199Ser nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0139 (betaine), 0.0206 (L-homocysteine) Homo sapiens
Pro197Ser nonsynonymous SNP identified using 240 DNA samples from four ethnic groups, Km (mM): 0.0213 (betaine), 0.0216 (L-homocysteine) Homo sapiens
T218M nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens
V155F nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens
V237M nonsynonymous SNP identified using 240 DNA samples from four ethnic groups Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
 L-homocysteine mutant Arg16Cys Homo sapiens
0.012
-
 betaine mutant Arg239Gln Homo sapiens
0.0139
-
 betaine mutant Arg16Cys Homo sapiens
0.0139
-
 betaine mutant Gly199Ser Homo sapiens
0.0158
-
 L-homocysteine mutant Arg239Gln Homo sapiens
0.0206
-
 L-homocysteine mutant Gly199Ser Homo sapiens
0.0213
-
 betaine mutant Pro197Ser Homo sapiens
0.0216
-
 L-homocysteine mutant Pro197Ser Homo sapiens
0.0227
-
 betaine wild-type Homo sapiens
0.029
-
 L-homocysteine wild-type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information no expression of BHMT2 protein is detected in liver or kidney although BHMT2 mRNA expression is observed Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homocysteine + betaine
-
 Homo sapiens L-methionine + dimethylglycine
-
 ?

Synonyms

Synonyms Comment Organism
betaine-homocysteine S-methyltransferase
-
 Homo sapiens
BHMT
-
 Homo sapiens
BHMT2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens