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Literature summary for 2.1.1.56 extracted from
- Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions (1975), J. Biol. Chem., 250, 9322-9329.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-homocysteine | - |
Vaccinia virus |  |
| sinefungin | - |
Vaccinia virus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| viral nucleocapsid | - |
Vaccinia virus | 19013 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Stokes radius determination | Vaccinia virus |
| 120000 | 129000 | gel filtration, sucrose density gradient centrifugation | Vaccinia virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + G(5')pppR-RNA | Vaccinia virus | - |
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | Vaccinia virus WR | - |
S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | - |
from infected HeLa cells | - |
| Vaccinia virus WR | - |
from infected HeLa cells | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| co-purifies with mRNA-guanylyltransferase | Vaccinia virus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, several months, stable | Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + G(5')pppR-RNA | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | - |
Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | transfers methyl group from S-adenosyl-L-methionine specifically to position 7 of terminal guanosine | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the methyl/guanylyltransferase complex on vaccinia virus dissociates into two subunits of MW 31400 and 95000 by action of SDS-PAGE | Vaccinia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vaccinia virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Vaccinia virus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000134 | - |
amino acid modified structure analogue of adenosyl-L-homocysteine | - |
Vaccinia virus | |
| 0.0000194 | - |
sinefungin | - |
Vaccinia virus | |
| 0.001 | - |
S-adenosyl-L-homocysteine | - |
Vaccinia virus | |
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