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Literature summary for 2.1.1.56 extracted from
- Methyltransferase and subunit association domains of vaccinia virus mRNA capping enzyme (1992), J. Biol. Chem., 267, 16424-16429.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli | Vaccinia virus |
| vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21 | Vaccinia virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of carboxyl-truncated D1 genes, amino acid 1-666 and 1-425: 347 residues are sufficient for activity | Vaccinia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| S-(3-deazaadenosyl)-L-homocysteine | - |
Vaccinia virus |  |
| S-(N6-Methyladenosyl)-L-homocysteine | - |
Vaccinia virus | |
| S-Adenosyl-D-homocysteine | - |
Vaccinia virus | |
| S-adenosyl-L-cysteine | - |
Vaccinia virus | |
| S-adenosyl-L-homocysteine | - |
Vaccinia virus | |
| S-Adenosyl-L-homocysteine sulfone | - |
Vaccinia virus | |
| S-adenosyl-L-homocysteine sulfoxide | - |
Vaccinia virus | |
| S-Aristeromycinyl-L-homocysteine | - |
Vaccinia virus | |
| S-Tubercidinyl-L-homocysteine | - |
Vaccinia virus | |
| sinefungin | - |
Vaccinia virus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| viral nucleocapsid | - |
Vaccinia virus | 19013 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
mutants | Vaccinia virus |
| 31000 | - |
1 * 31000 + 1 * 95000, SDS-PAGE | Vaccinia virus |
| 95000 | - |
1 * 31000 + 1 * 95000, SDS-PAGE | Vaccinia virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + G(5')pppR-RNA | Vaccinia virus | the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | Vaccinia virus | one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] | RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit | Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + G(5')pppR-RNA | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 31000 + 1 * 95000, SDS-PAGE | Vaccinia virus |
| More | subunit organisation of the mRNA capping enzyme complex | Vaccinia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vaccinia virus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000018 | - |
amino acid modified structure analogue of adenosyl-L-homocysteine | - |
Vaccinia virus | |
| 0.0000075 | - |
sinefungin | - |
Vaccinia virus | |
| 0.00053 | - |
S-adenosyl-L-homocysteine | - |
Vaccinia virus | |
| 0.0012 | - |
S-Adenosyl-L-homocysteine sulfone | - |
Vaccinia virus | |
| 0.0012 | - |
S-adenosyl-L-homocysteine sulfoxide | - |
Vaccinia virus | |
| 0.0012 | - |
S-Tubercidinyl-L-homocysteine | - |
Vaccinia virus | |
| 0.0021 | - |
S-(3-deazaadenosyl)-L-homocysteine | - |
Vaccinia virus | |
| 0.0026 | - |
S-Aristeromycinyl-L-homocysteine | - |
Vaccinia virus | |
| 0.0046 | - |
S-(3'-aminoadenosyl)-L-homocysteine | - |
Vaccinia virus | |
| 0.009 | - |
S-(N6-Methyladenosyl)-L-homocysteine | - |
Vaccinia virus | |
| 0.014 | - |
S-Adenosyl-D-homocysteine | - |
Vaccinia virus | |
| 0.058 | - |
S-adenosyl-L-cysteine | - |
Vaccinia virus | |
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