Any feedback?
Literature summary for 2.1.1.56 extracted from
- Purification of mRNA guanylyltransferase from vaccinia virions (1978), J. Biol. Chem., 253, 4481-4489.
General Stability
| General Stability | Organism |
|---|---|
| gel electrophoresis inactivates | Vaccinia virus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| viral nucleocapsid | - |
Vaccinia virus | 19013 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | 129000 | glycerol gradient centrifugation | Vaccinia virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | - |
from infected HeLa cells | - |
| Vaccinia virus WR | - |
from infected HeLa cells | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| co-purifies with mRNA-guanylyltransferase | Vaccinia virus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 16 h at pH 7.5 | Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + G(5')pppR-RNA | R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? |  |
| S-adenosyl-L-methionine + G(5')pppR-RNA | R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs | Vaccinia virus WR | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
inactivation after 16 h at 0°C | Vaccinia virus |
| 7.5 | - |
16 h stable at 0°C | Vaccinia virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
