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Literature summary for 2.1.1.56 extracted from
- The mRNA (guanine-7-)methyltransferase domain of the vaccinia virus mRNA capping enzyme. Expression in Escherichia coli and structural and kinetic comparison to the intact capping enzyme (1994), J. Biol. Chem., 269, 14974-14981.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| D1 fragment, amino acids 498 to 844 | Vaccinia virus |
| individual and coexpression of His-tagged D1 large subunit fragment, being the methyltransferase domain of the enzyme, and of D12 small subunit in Escherichia coli | Vaccinia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strong | Vaccinia virus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
S-adenosyl-L-methionine | intact mRNA capping enzyme and isolated methyltransferase domain | Vaccinia virus | |
| 0.033 | - |
GTP | intact mRNA capping enzyme and isolated methyltransferase domain | Vaccinia virus | |
| 0.036 | - |
GTP | isolated methyltransferase domain | Vaccinia virus | |
| 0.085 | - |
G(5')pppG | intact mRNA capping enzyme | Vaccinia virus | |
| 0.101 | - |
G(5')pppG | isolated methyltransferase domain | Vaccinia virus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
small subunit of mRNA capping enzyme , gel filtration, SDS-PAGE | Vaccinia virus |
| 97000 | - |
large subunit of mRNA capping enzyme, gel filtration, SDS-PAGE | Vaccinia virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| methyltransferase domain, and small subunit of mRNA capping enzyme, recombinant from E. coli | Vaccinia virus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] | RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit | Vaccinia virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0196 | - |
purified D1 fragment, residues 498-844 | Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit | Vaccinia virus | ? | - |
? | |
| S-adenosyl-L-methionine + G(5')pppR-RNA | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
| S-adenosyl-L-methionine + GpppG | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7GpppG | - |
? | |
| S-adenosyl-L-methionine + GTP | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7GTP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | isolated methyltransferase domain shows a lower but kinetically equivalent activity than the complete enzyme, which is highly enhanced by association with the D12 subunit | Vaccinia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vaccinia virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Vaccinia virus |
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