Literature summary for 2.1.1.56 extracted from

Dong, H.; Chang, D.C.; Xie, X.; Toh, Y.X.; Chung, K.Y.; Zou, G.; Lescar, J.; Lim, S.P.; Shi, P.Y.
Biochemical and genetic characterization of dengue virus methyltransferase (2010), Virology, 405, 568-578.

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Cloned(Commentary)

Cloned (Comment)	Organism
the MTase domain, representing the N-terminal 272 amino acids of DENV-4 NS5 is cloned and purified	Dengue virus

Protein Variants

Protein Variants	Comment	Organism
D146A	mutant completely loses the N-7 activity (methylation of N-7 position of guanine) and 2'-O methylation activity. Mutation defective in N-7 methylation is lethal for DNV replication	Dengue virus
E217A	mutants retains 59% of wild-type N-7 (methylation of N-7 position of guanine) activity	Dengue virus
K181A	mutants retains 16% of wild-type N-7 (methylation of N-7 position of guanine) activity	Dengue virus
K61A	mutants retains 34% of wild-type N-7 (methylation of N-7 position of guanine) activity	Dengue virus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	SDS-PAGE	Dengue virus

Organism

Organism	UniProt	Comment	Textmining
Dengue virus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme sequentially methylates the guanine N-7 and ribose 2'-O positions of viral RNA cap. The order of two methylations is determined by the preference of 2'-O methylation for substrate m7GpppA-RNA to GpppA-RNA, and the 2'-O methylation is not absolutely dependent on the prior N-7 methylation	Dengue virus	?	-	?
S-adenosyl-L-methionine + GpppA-RNA	-	Dengue virus	S-adenosyl-L-homocysteine + m7GpppA-RNA	-	?

Synonyms

Synonyms	Comment	Organism
DENV methyltransferase	-	Dengue virus
DENV-4 NS5	-	Dengue virus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Dengue virus

General Information

General Information	Comment	Organism
malfunction	mutation defective in N-7 methylation but not 2-O'-methylation is lethal for DNV replication	Dengue virus

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Release 2023.1 (January 2023)