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Literature summary for 2.1.1.56 extracted from

  • Jin, X.; Chen, Y.; Sun, Y.; Zeng, C.; Wang, Y.; Tao, J.; Wu, A.; Yu, X.; Zhang, Z.; Tian, J.; Guo, D.
    Characterization of the guanine-N7 methyltransferase activity of coronavirus nsp14 on nucleotide GTP (2013), Virus Res., 176, 45-52.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C382Y 15-50% of wild-type activity Severe acute respiratory syndrome-related coronavirus
C414R mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
C416R mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
D331A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
D331Y mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
D352A 15-50% of wild-type activity Severe acute respiratory syndrome-related coronavirus
F73A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
G333A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
I332A 15-50% of wild-type activity Severe acute respiratory syndrome-related coronavirus
K336A 15-50% of wild-type activity Severe acute respiratory syndrome-related coronavirus
L419R 15-50% of wild-type activity Severe acute respiratory syndrome-related coronavirus
P335A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
R310A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
R84A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
T428A 15-50% of wild-type activity Severe acute respiratory syndrome-related coronavirus
W86A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
Y368A mutation abolishes the N7-MTase activity on GTP Severe acute respiratory syndrome-related coronavirus
Y420A 15-50% of wild-type activity Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus P0C6X7
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates: ATP, CTP, UTP, dATP, dCTP, dUTP or cap analog m7GpppA Severe acute respiratory syndrome-related coronavirus ?
-
?
S-adenosyl-L-methionine + dGTP and G(5')pppG, best substrates Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7dGTP
-
?
S-adenosyl-L-methionine + G(5')pppA
-
Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppA
-
?
S-adenosyl-L-methionine + G(5')pppG and dGTP, best substrates Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppG
-
?
S-adenosyl-L-methionine + GTP
-
Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7GTP
-
?
S-adenosyl-L-methionine + m7G(5')pppG
-
Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + ?
-
?