Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | |
1.33 | - |
[cytochrome c]-L-lysine | of horse heart | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytochrome c L-lysine | Saccharomyces cerevisiae | in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | Saccharomyces cerevisiae | regulation of enzyme and cytochrome c substrate is not in parallel manner | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000117 | - |
partially purified enzyme | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, pH 7.0, crude extract supernatant, stable | Saccharomyces cerevisiae |
purified enzyme is stabilized by addition of 66% ammonium sulfate during storage | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytochrome c L-lysine | specific for unmethylated cytochrome c | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | a single lysine residue at position 72 of horse heart cytochrome c is methylated | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | specific for unmethylated cytochrome c | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | 9:17:74 | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | specific for unmethylated cytochrome c | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | horse heart cytochrome c-72 | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | a single lysine residue at position 72 of horse heart cytochrome c is methylated | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | horse heart cytochrome c-72 | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | 9:17:74 | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | horse heart cytochrome c-72 | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | overview: substrate activity of various cytochromes c | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | a single lysine residue at position 72 of horse heart cytochrome c is methylated | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | overview: substrate activity of various cytochromes c | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | 9:17:74 | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | overview: substrate activity of various cytochromes c | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | in vivo methylation of cytochrome c in yeast occurs probably before cytochrome c is attached to the mitochondria | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | regulation of enzyme and cytochrome c substrate is not in parallel manner | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + [cytochrome c]-L-lysine | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Saccharomyces cerevisiae |