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Literature summary for 2.1.1.6 extracted from
- Purification and characterization of Streptomyces griseus catechol O-methyltransferase (2000), Appl. Environ. Microbiol., 66, 4877-4882.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | complete inhibition at 1 mM | Streptomyces griseus |  |
| homocysteine | - |
Streptomyces griseus | |
| N-ethylmaleimide | complete inhibition at 1 mM | Streptomyces griseus | |
| p-chloromercuribenzoate | complete inhibition at 1 mM | Streptomyces griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | assay method | Streptomyces griseus | |
| 0.5 | - |
6,7-dihydroxycoumarin | - |
Streptomyces griseus | |
| 0.6 | - |
S-adenosyl-L-methionine | - |
Streptomyces griseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Streptomyces griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Streptomyces griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
S-COMT | - |
| Streptomyces griseus S-COMT | - |
S-COMT | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrocatechol + S-adenosyl-L-methionine | - |
Streptomyces griseus | S-adenosyl-L-homocysteine + ? | - |
? | |
| 4-nitrocatechol + S-adenosyl-L-methionine | - |
Streptomyces griseus S-COMT | S-adenosyl-L-homocysteine + ? | - |
? | |
| 6,7-dihydroxycoumarin + S-adenosyl-L-methionine | - |
Streptomyces griseus | S-adenosyl-L-homocysteine + ? | - |
? | |
| 6,7-dihydroxycoumarin + S-adenosyl-L-methionine | - |
Streptomyces griseus S-COMT | S-adenosyl-L-homocysteine + ? | - |
? | |
| caffeic acid + S-adenosyl-L-methionine | - |
Streptomyces griseus | S-adenosyl-L-homocysteine + ? | - |
? | |
| caffeic acid + S-adenosyl-L-methionine | - |
Streptomyces griseus S-COMT | S-adenosyl-L-homocysteine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE, gel filtration | Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Streptomyces griseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces griseus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.224 | - |
homocysteine | - |
Streptomyces griseus | |
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Release 2023.1 (January 2023)
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