Cloned (Comment) | Organism |
---|---|
expression of the human soluble and membrane-bound COMTs (S-COMT and MBCOMT, respectively) in Escherichia coli. The two recombinant human COMTs are functionally active, with catalytic and kinetic properties nearly identical to that of crude or purified enzymes prepared from human tissues or cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
quercetin | S-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0015 | - |
fisetin | S-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0022 | - |
catechin | MB-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0032 | - |
2-hydroxyestradiol | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0036 | - |
2-hydroxyestradiol | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0043 | - |
epicatechin | MB-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0045 | - |
4-hydroxyestradiol | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0058 | - |
S-adenosyl-L-methionine | cosubstrate: 4-hydroxyestradiol, MB-COMT | Homo sapiens | |
0.0064 | - |
4-hydroxyestradiol | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0089 | - |
catechin | S-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0144 | - |
S-adenosyl-L-methionine | cosubstrate: 2-hydroxyestradiol, MB-COMT | Homo sapiens | |
0.017 | - |
dopamine | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0257 | - |
epicatechin | S-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0288 | - |
epinephrine | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.0306 | - |
norepinephrine | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.1136 | - |
S-adenosyl-L-methionine | cosubstrate: 2-hydroxyestradiol, S-COMT | Homo sapiens | |
0.1453 | - |
S-adenosyl-L-methionine | cosubstrate: 4-hydroxyestradiol, S-COMT | Homo sapiens | |
0.224 | - |
norepinephrine | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.2632 | - |
dopamine | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.3947 | - |
epinephrine | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound enzyme form MB-COMT | Homo sapiens | 16020 | - |
soluble | soluble enzyme form S-COMT | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 24000, S-COMT, SDS-PAGE | Homo sapiens |
30000 | - |
x * 30000, MB-COMT, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
recombinant | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyestradiol + S-adenosyl-L-methionine | MB-COMT methylates both 2-hydroxyestradiol and 4-hydroxyestradiol at comparable rates | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
2-hydroxyestradiol + S-adenosyl-L-methionine | S-COMT methylates 2-hydroxyestradiol roughly twice as fast as it methylates 4-hydroxyestradiol | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
4-hydroxyestradiol + S-adenosyl-L-methionine | MB-COMT methylates both 2-hydroxyestradiol and 4-hydroxyestradiol at comparable rates | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
4-hydroxyestradiol + S-adenosyl-L-methionine | S-COMT methylates 2-hydroxyestradiol roughly twice as fast as it methylates 4-hydroxyestradiol | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
dopamine + S-adenosyl-L-methionine | membrane-bound enzyme form MB-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
dopamine + S-adenosyl-L-methionine | soluble enzyme form S-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
epinephrine + S-adenosyl-L-methionine | membrane-bound enzyme form MB-COMT | Homo sapiens | metanephrine + S-adenosyl-L-homocysteine | - |
? | |
epinephrine + S-adenosyl-L-methionine | soluble enzyme form S-COMT | Homo sapiens | metanephrine + S-adenosyl-L-homocysteine | - |
? | |
S-adenosyl-L-methionine + catechin | membrane-bound enzyme form MB-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + catechin | soluble enzyme form S-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + epicatechin | membrane-bound enzyme form MB-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + epicatechin | soluble enzyme form S-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + fisetin | membrane-bound enzyme form MB-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + fisetin | soluble enzyme form S-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + norepinephrine | membrane-bound enzyme form MB-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + norepinephrine | soluble enzyme form S-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + quercetin | membrane-bound enzyme form MB-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + quercetin | soluble enzyme form S-COMT | Homo sapiens | S-adenosyl-L-homocysteine + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 24000, S-COMT, SDS-PAGE | Homo sapiens |
? | x * 30000, MB-COMT, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MB-COMT | membrane-bound enzyme form | Homo sapiens |
S-COMT | soluble enzyme form | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
MB-COMT protein is quite stable at 37°C | Homo sapiens |
37 | - |
S-COMT protein is quite stable at 37°C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
4-hydroxyestradiol | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.04 | - |
norepinephrine | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.077 | - |
4-hydroxyestradiol | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.085 | - |
2-hydroxyestradiol | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.085 | - |
epinephrine | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.088 | - |
dopamine | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.17 | - |
epicatechin | S-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.18 | - |
norepinephrine | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.18 | - |
catechin | S-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.19 | - |
dopamine | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.25 | - |
epinephrine | MB-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens | |
0.26 | - |
catechin | MB-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.315 | - |
epicatechin | MB-COMT, 0.25 mM S-adenosyl-L-methionine | Homo sapiens | |
0.99 | - |
2-hydroxyestradiol | S-COMT, 0.1 mM S-adenosyl-L-methionine | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | methylation of 2-hydroxyestradiol or 4-hydroxyestradiol by MB-COMT | Homo sapiens |
7 | 8 | methylation of 2-hydroxyestradiol or 4-hydroxyestradiol by S-COMT | Homo sapiens |