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Literature summary for 2.1.1.62 extracted from

  • Keith, J.M.; Ensinger, M.J.; Moss, B.
    HeLa cell RNA (2-O-methyladenosine-N6-)-methyltransferase specific for the capped 5-end of messenger RNA (1978), J. Biol. Chem., 253, 5033-5041.
    View publication on PubMed
Search for more literature for 2.1.1.62

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 sucrose density gradient Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA Homo sapiens involved in post-transcriptional modification of 5'-end of messenger-RNA S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA mRNA containing an N6,2'-O-dimethyladenosine cap ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human, HeLa S3 cells
-

Purification (Commentary)

Purification (Comment) Organism
partially Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000418
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, 0.21 M (NH4)2SO4, stable for longer than 5 months Homo sapiens
0°C, 0.21 M (NH4)2SO4, stable for 4-5 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrates are the oligonucleotides m7G(5')pppAm, m7G(5')pppA, m7G(5')pppAmpN, no methyl group acceptors are brome mosaic virus RNA, E. coli tRNA and rRNA and yeast tRNA Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + G(5')pppAm-mRNA poor substrate Homo sapiens S-adenosyl-L-homocysteine + G(5')pppm6Am-mRNA
-
 ?
S-adenosyl-L-methionine + m7G(5')pppA-mRNA
-
 Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppm6A-mRNA
-
 ?
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA methyl group acceptor is methylated or unmethylated vaccinia virus RNA Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA mRNA containing an N6,2'-O-dimethyladenosine cap ?
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA involved in post-transcriptional modification of 5'-end of messenger-RNA Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA mRNA containing an N6,2'-O-dimethyladenosine cap ?
S-adenosyl-L-methionine + pppA-mRNA
-
 Homo sapiens S-adenosyl-L-homocysteine + pppm6A-mRNA
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Homo sapiens