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Literature summary for 2.1.1.63 extracted from
- Alkylation damage repair protein O6-alkylguanine-DNA alkyltransferase from the hyperthermophiles Aquifex aeolicus and Archaeoglobus fulgidus (2003), Biochem. J., 375, 449-455.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography | Aquifex aeolicus |
| expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography | Archaeoglobus fulgidus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O6-benzylguanine | - |
Aquifex aeolicus |  |
| O6-benzylguanine | - |
Archaeoglobus fulgidus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16718 | - |
x * 16718, calculation from nucleotide sequence | Archaeoglobus fulgidus |
| 23000 | - |
- |
Aquifex aeolicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA containing 6-O-methylguanine + [protein]-L-cysteine | Aquifex aeolicus | DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
| DNA containing 6-O-methylguanine + [protein]-L-cysteine | Archaeoglobus fulgidus | DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O67467 | - |
- |
| Archaeoglobus fulgidus | O27970 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography | Aquifex aeolicus |
| expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography | Archaeoglobus fulgidus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stored at, storage at -20°C or at -80°C results in protein precipitation | Aquifex aeolicus |
| 4°C, stored at, storage at -20°C or at -80°C results in protein precipitation | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Aquifex aeolicus | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
| DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Archaeoglobus fulgidus | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
| DNA containing 6-O-methylguanine + [protein]-L-cysteine | DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents | Aquifex aeolicus | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
| DNA containing 6-O-methylguanine + [protein]-L-cysteine | DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents | Archaeoglobus fulgidus | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16718, calculation from nucleotide sequence | Archaeoglobus fulgidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| O6-alkylguanine-DNA alkyltransferase | - |
Aquifex aeolicus |
| O6-alkylguanine-DNA alkyltransferase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Aquifex aeolicus |
| 80 | - |
- |
Archaeoglobus fulgidus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | 80°C: optimum, 100°C: substantial repair activity at | Archaeoglobus fulgidus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life: 12 h | Aquifex aeolicus |
| 60 | - |
half-life: 24 h | Archaeoglobus fulgidus |
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