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Literature summary for 2.1.1.63 extracted from

  • Fang, Q.; Kanugula, S.; Tubbs, J.L.; Tainer, J.A.; Pegg, A.E.
    Repair of O4-alkylthymine by O6-alkylguanine-DNA alkyltransferases (2009), J. Biol. Chem., 285, 8185-8195.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.63

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 Blue cells Escherichia coli
expressed in Escherichia coli XL-1 Blue cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construct human AGT-03 (where AGT sequence -V149CSSGAVGN157- is replaced with the corresponding Ogt -I143GRNGTMTG151-), exhibits enhanced DNA containing 4-O-methylthymine repair activity in vitro compared to wild type AGT Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens P16455
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity column chromatography Escherichia coli
immobilized metal affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA containing 4-O-methylthymine + [protein]-L-cysteine
-
 Escherichia coli DNA lacking 4-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Escherichia coli DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
additional information human AGT acts poorly on DNA containing 4-O-methylthymine Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
AGT
-
 Homo sapiens
O6-alkylguanine-DNA alkyltransferase
-
 Escherichia coli
O6-alkylguanine-DNA alkyltransferase
-
 Homo sapiens
OGT
-
 Escherichia coli