Literature summary for 2.1.1.63 extracted from

Miggiano, R.; Casazza, V.; Garavaglia, S.; Ciaramella, M.; Perugino, G.; Rizzi, M.; Rossi, F.
Biochemical and structural studies of the Mycobacterium tuberculosis O6-methylguanine methyltransferase and mutated variants (2013), J. Bacteriol., 195, 2728-2736.

Search for more literature for 2.1.1.63

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), and 6% (w/v) polyethylene glycol 8000	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
R37L	the mutant exhibits a 10fold-lower affinity for double stranded methylated DNA compared to the wild type enzyme	Mycobacterium tuberculosis
T15S	the mutant exhibits a 2fold-lower affinity for double stranded methylated DNA compared to the wild type enzyme	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17844	-	x * 17844, calculated from amino acid sequence	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA (containing 6-O-methylguanine) + protein L-cysteine	Mycobacterium tuberculosis	-	DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WJW5	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrapQ column chromatography, MonoQ column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA (containing 6-O-methylguanine) + protein L-cysteine	-	Mycobacterium tuberculosis	DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine	-	?

Subunits

Subunits	Comment	Organism
?	x * 17844, calculated from amino acid sequence	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
O6-methylguanine methyltransferase	-	Mycobacterium tuberculosis
OGT	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)