Literature summary for 2.1.1.64 extracted from

Leppik, R.A.; Stoobant, P.; Shineberg, B.; Young, I.G.; Gibson, F.
Membrane-associated reactions in ubiquinone biosynthesis. 2-Octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone methyltransferase (1976), Biochim. Biophys. Acta, 428, 146-156.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	the optimum ratio of dithiothreitol to divalent metal ion is about 10:1. The maximal methyltransferase activity is achieved at 10 mM dithiothreitol and 1 mM Zn2+	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	associated. In vivo, the methyltransferase reaction probably occurs at the internal surface of the cytoplasmic membrane	Escherichia coli	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	absolute requirement for a divalent metal ion. 1 mM Cd2+ stimulates 14fold	Escherichia coli
Co2+	absolute requirement for a divalent metal ion. 1 mM Co2+ stimulates 67fold	Escherichia coli
Cu2+	absolute requirement for a divalent metal ion. 1 mM Cu2+ stimulates 21fold	Escherichia coli
Fe2+	absolute requirement for a divalent metal ion. 1 mM Fe2+ stimulates 63fold	Escherichia coli
Mg2+	absolute requirement for a divalent metal ion. 1 mM Mg2+ stimulates about 3fold	Escherichia coli
Mn2+	absolute requirement for a divalent metal ion. 1 mM Mn2+ stimulates 11fold	Escherichia coli
Ni2+	absolute requirement for a divalent metal ion. 1 mM Ni2+ stimulates 24fold	Escherichia coli
Zn2+	absolute requirement for a divalent metal ion. Zn2+ is most effective. 1 mM Zn2+ stimulates over 100fold	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	gel filtration	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + 3-demethylubiquinol-8	Escherichia coli	involved in biosynthesis of ubiquinone-8	S-adenosyl-L-homocysteine + ubiquinol-8	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 3-demethylubiquinol-8	involved in biosynthesis of ubiquinone-8	Escherichia coli	S-adenosyl-L-homocysteine + ubiquinol-8	-	?
S-adenosyl-L-methionine + 3-demethylubiquinol-8	i.e. 2-all-trans-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol	Escherichia coli	S-adenosyl-L-homocysteine + ubiquinol-8	-	?

Synonyms

Synonyms	Comment	Organism
2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone methyltransferase	-	Escherichia coli
OMHMB methyltransferase	-	Escherichia coli
S-adenosyl-L-methionine:2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone-O-methyltransferase	-	Escherichia coli
UbiG	-	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)