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Literature summary for 2.1.1.64 extracted from
- Crystallization and preliminary crystallographic studies of UbiG, an O-methyltransferase from Escherichia coli (2011), Acta Crystallogr. Sect. F, 67, 727-729.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His6-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using either 0.1 M HEPES pH 7.5 and 20% polyethylene glycol 10000 or 10% (v/v) 2-methyl-2,4-pentanediol and 0.1 M HEPES pH 7.0 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26600 | - |
x * 26600, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, DEAE column chromatography, and Superdex 200 gel filtration | Escherichia coli |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26600, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| O-methyltransferase | - |
Escherichia coli |
| UbiG | - |
Escherichia coli |
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