Application | Comment | Organism |
---|---|---|
medicine | thiopurine S-methyltransferase is an enzyme responsible for the detoxification of the widely used thiopurine drugs | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the pCR2.1-TOPO vector, used as a template to generate point mutations, the variants are sequenced and cloned into the mammalian expression vector pCMV6-XL5, into the pENTR/D-TOPO vector and subsequently into the pcDNA-DEST40 vector | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A154T | variant TPMT*3B | Homo sapiens |
A154T/Y240C | variant TPMT*3A | Homo sapiens |
A167G | variant TPMT*23 | Homo sapiens |
A80P | variant TPMT*2 | Homo sapiens |
C132Y | variant TPMT*11 | Homo sapiens |
E28V | variant TPMT*13 | Homo sapiens |
G144R | variant TPMT*10 | Homo sapiens |
G36S | variant TPMT*24 | Homo sapiens |
G71R | variant TPMT*18 | Homo sapiens |
H227Q | variant TPMT*7 | Homo sapiens |
K119T | variant TPMT*9 | Homo sapiens |
K122T | variant TPMT*19 | Homo sapiens |
K238E | variant TPMT*20 | Homo sapiens |
L49S | variant TPMT*5 | Homo sapiens |
L69V | variant TPMT*21 | Homo sapiens |
M1V | variant TPMT*14 | Homo sapiens |
Q42E | variant TPMT*17 | Homo sapiens |
R163H | variant TPMT*16 | Homo sapiens |
R163P | variant TPMT*22 | Homo sapiens |
R215H | variant TPMT*8 | Homo sapiens |
S125L | variant TPMT*12 | Homo sapiens |
Y180F | variant TPMT*6 | Homo sapiens |
Y240C | variant TPMT*3C | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
6-thioguanine | mutant R215H, variant TPMT*8 | Homo sapiens | |
0.076 | - |
6-thioguanine | mutant K119T, variant TPMT*9 | Homo sapiens | |
0.08 | - |
6-thioguanine | wild-type, variant TPMT*1 | Homo sapiens | |
0.098 | - |
6-thioguanine | mutant A167G, variant TPMT*23 | Homo sapiens | |
0.125 | - |
6-thioguanine | mutant G144R, variant TPMT*10 | Homo sapiens | |
0.127 | - |
6-thioguanine | mutant S125L, variant TPMT*12 | Homo sapiens | |
0.151 | - |
6-thioguanine | mutant A80P, variant TPMT*2 | Homo sapiens | |
0.156 | - |
6-thioguanine | mutant Y240C, variant TPMT*3C | Homo sapiens | |
0.186 | - |
6-thioguanine | mutant K122T, variant TPMT*19 | Homo sapiens | |
0.353 | - |
6-thioguanine | mutant K238E, variant TPMT*20 | Homo sapiens | |
0.485 | - |
6-thioguanine | mutant Y180F, variant TPMT*6 | Homo sapiens | |
0.55 | - |
6-thioguanine | mutant E28V, variant TPMT*13 | Homo sapiens | |
0.6 | - |
6-thioguanine | mutant R163H, variant TPMT*16 | Homo sapiens | |
1.027 | - |
6-thioguanine | mutant H227Q, variant TPMT*7 | Homo sapiens | |
1.094 | - |
6-thioguanine | mutant G36S, variant TPMT*24 | Homo sapiens | |
1.267 | - |
6-thioguanine | mutant Q42E, variant TPMT*17 | Homo sapiens | |
1.45 | - |
6-thioguanine | mutant C132Y, variant TPMT*11 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + a thiopurine | Homo sapiens | - |
S-adenosyl-L-homocysteine + a thiopurine S-methylether | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 6-mercaptopurine | - |
Homo sapiens | S-adenosyl-L-homocysteine + 6-methylmercaptopurine | - |
? | |
S-adenosyl-L-methionine + 6-thioguanine | - |
Homo sapiens | S-adenosyl-L-homocysteine + 6-methylthioguanine | - |
? | |
S-adenosyl-L-methionine + 6-thioguanine monophosphate | - |
Homo sapiens | S-adenosyl-L-homocysteine + 6-methylthioguanine monophosphate | - |
? | |
S-adenosyl-L-methionine + 6-thioinosine monophosphate | - |
Homo sapiens | S-adenosyl-L-homocysteine + 6-methylthioinosine monophosphate | - |
? | |
S-adenosyl-L-methionine + a thiopurine | - |
Homo sapiens | S-adenosyl-L-homocysteine + a thiopurine S-methylether | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thiopurine S-methyltransferase | - |
Homo sapiens |
TPMT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay of thiopurine S-methyltransferase activity in COS-7 cells | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
enzyme assay of thiopurine S-methyltransferase activity in COS-7 cells | Homo sapiens |