Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.68 extracted from

  • Chen, L.; Auh, C.K.; Dowling, P.; Bell, J.; Lehmann, D.; Wang, Z.Y.
    Transgenic down-regulation of caffeic acid O-methyltransferase (COMT) led to improved digestibility in tall fescue (Festuca arundinacea) (2004), Funct. Plant Biol., 31, 235-245.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Transgenic Festuca arundinacea plants carrying either sense or antisense COMT gene constructs are obtained by microprojectile bombardment of single-genotype-derived embryogenic suspension cells. Two co-suppressed transgenic lines are down-regulated in their lignin biosynthesis. These down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility Lolium arundinaceum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0109
-
5-hydroxyferulic acid pH 7.5, 30°C Lolium arundinaceum
0.0148
-
caffeoyl aldehyde pH 7.5, 30°C Lolium arundinaceum
0.0185
-
5-hydroxyconiferaldehyde pH 7.5, 30°C Lolium arundinaceum
0.027
-
caffeoyl alcohol pH 7.5, 30°C Lolium arundinaceum
0.0497
-
caffeic acid pH 7.5, 30°C Lolium arundinaceum
0.068
-
5-hydroxyconiferyl alcohol pH 7.5, 30°C Lolium arundinaceum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid Lolium arundinaceum key lignin biosynthetic enzyme. Down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
-
?

Organism

Organism UniProt Comment Textmining
Lolium arundinaceum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid
-
Lolium arundinaceum S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
-
?
S-adenosyl-L-methionine + 3,4,5-trihydroxycinnamic acid key lignin biosynthetic enzyme. Down-regulated transgenic plants show substantially reduced levels of transcripts, significantly reduced enzymatic activities, significantly decreased lignin content, apparently altered lignin composition and significantly increased digestibility Lolium arundinaceum S-adenosyl-L-homocysteine + 4,5-dihydroxy-3-methoxycinnamic acid
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
-
Lolium arundinaceum S-adenosyl-L-homocysteine + 5-methoxyconiferaldehyde
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferyl alcohol
-
Lolium arundinaceum S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
Lolium arundinaceum S-adenosyl-L-homocysteine + sinapic acid
-
?
S-adenosyl-L-methionine + caffeic acid
-
Lolium arundinaceum S-adenosyl-L-homocysteine + ferulic acid
-
r
S-adenosyl-L-methionine + caffeoyl alcohol
-
Lolium arundinaceum S-adenosyl-L-homocysteine + coniferyl alcohol
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
-
Lolium arundinaceum S-adenosyl-L-homocysteine + ferulic aldehyde
-
?

Synonyms

Synonyms Comment Organism
COMT
-
Lolium arundinaceum