Literature summary for 2.1.1.68 extracted from

Louie, G.V.; Bowman, M.E.; Tu, Y.; Mouradov, A.; Spangenberg, G.; Noel, J.P.
Structure-function analyses of a caffeic acid O-methyltransferase from perennial ryegrass reveal the molecular basis for substrate preference (2010), Plant Cell, 22, 4114-4127.

Crystallization (Commentary)

Crystallization (Comment)	Organism
x-ray crystallographic structures of Lp OMT1 are reported in open conformational state, apo- and holoenzyme forms and, most significantly, in a closed conformational state complexed with the products S-adenosyl-L-homocysteine and sinapaldehyde. The product-bound complex reveals the postmethyl-transfer organization of COMTs catalytic groups with reactant molecules and the fully formed phenolic-ligand binding site. The core scaffold of the phenolic ligand forges a hydrogen-bonding network involving the 4-hydroxy group that anchors the aromatic ring and thereby permits only metahydroxyl groups to be positioned for transmethylation	Lolium perenne

Crystallization (Comment)

Organism

x-ray crystallographic structures of Lp OMT1 are reported in open conformational state, apo- and holoenzyme forms and, most significantly, in a closed conformational state complexed with the products S-adenosyl-L-homocysteine and sinapaldehyde. The product-bound complex reveals the postmethyl-transfer organization of COMTs catalytic groups with reactant molecules and the fully formed phenolic-ligand binding site. The core scaffold of the phenolic ligand forges a hydrogen-bonding network involving the 4-hydroxy group that anchors the aromatic ring and thereby permits only metahydroxyl groups to be positioned for transmethylation

Lolium perenne

Organism

Organism	UniProt	Comment	Textmining
Lolium perenne	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde	-	Lolium perenne	?	-	?
S-adenosyl-L-methionine + 5-hydroxyferulic acid	-	Lolium perenne	S-adenosyl-L-homocysteine + sinapic acid	-	?
S-adenosyl-L-methionine + caffeic acid	-	Lolium perenne	?	-	?
S-adenosyl-L-methionine + caffeoyl alcohol	-	Lolium perenne	?	-	?
S-adenosyl-L-methionine + caffeoyl aldehyde	-	Lolium perenne	?	-	?

Synonyms

Synonyms	Comment	Organism
Lp OMT1	-	Lolium perenne

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Lolium perenne

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Lolium perenne