Crystallization (Comment) | Organism |
---|---|
x-ray crystallographic structures of Lp OMT1 are reported in open conformational state, apo- and holoenzyme forms and, most significantly, in a closed conformational state complexed with the products S-adenosyl-L-homocysteine and sinapaldehyde. The product-bound complex reveals the postmethyl-transfer organization of COMTs catalytic groups with reactant molecules and the fully formed phenolic-ligand binding site. The core scaffold of the phenolic ligand forges a hydrogen-bonding network involving the 4-hydroxy group that anchors the aromatic ring and thereby permits only metahydroxyl groups to be positioned for transmethylation | Lolium perenne |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lolium perenne | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde | - |
Lolium perenne | ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | - |
Lolium perenne | S-adenosyl-L-homocysteine + sinapic acid | - |
? | |
S-adenosyl-L-methionine + caffeic acid | - |
Lolium perenne | ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl alcohol | - |
Lolium perenne | ? | - |
? | |
S-adenosyl-L-methionine + caffeoyl aldehyde | - |
Lolium perenne | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lp OMT1 | - |
Lolium perenne |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Lolium perenne |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Lolium perenne |