Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.72 extracted from

  • Malygin, E.G.; Lindstrom, W.M., Jr.; Zinoviev, V.V.; Evdokimov, A.A.; Schlagman, S.L.; Reich, N.O.; Hattman, S.
    Bacteriophage T4Dam (DNA-(adenine-N6)-methyltransferase): evidence for two distinct stages of methylation under single turnover conditions (2003), J. Biol. Chem., 278, 41749-41755.
    View publication on PubMed
Search for more literature for 2.1.1.72

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state and pre-steady-state kinetics, single turnover methylation kinetics Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Tequatrovirus T4 unmethylated or hemimethylated DNA S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA two distinct stages of methylation under single turnover conditions Tequatrovirus T4
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme and S-adenosyl-L-methionine form a catalytically active complex Tequatrovirus T4 ?
-
 ?
S-adenosyl-L-methionine + (CGG*CCG)73 oligodeoxynucleotide duplexes 5'-GATC/5'-GATC
-
 Tequatrovirus T4 ?
-
 ?
S-adenosyl-L-methionine + DNA adenine unmethylated or hemimethylated DNA Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
S-adenosyl-L-methionine + DNA adenine unmethylated or hemimethylated DNA, two distinct stages of methylation under single turnover conditions Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
S-adenosyl-L-methionine + oligodeoxynucleotide duplexes substrate contains one single specific recognition site as 5'-GATC/5'-GATC, or modified variants Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Subunits

Subunits Comment Organism
monomer enzyme and S-adenosyl-L-methionine form a catalytically active complex Tequatrovirus T4

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Tequatrovirus T4
0.21
-
 oligodeoxynucleotide duplexes 5'-GATC/5'-GATC
-
 Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Tequatrovirus T4