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Literature summary for 2.1.1.72 extracted from

  • Zinoviev, V.V.; Evdokimov, A.A.; Hattman, S.; Malygin, E.G.
    Molecular enzymology of phage T4 Dam DNA methyltransferase (2004), Mol. Biol., 38, 737-751.
No PubMed abstract available
Search for more literature for 2.1.1.72

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pre-steady-state and steady-state kinetics, reaction kinetics with diverse DNA duplex substrates, role of individual elements of the recognition site, kinetic reaction scheme assuming that the enzyme is isomerized into a catalytically active form, overview Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 sucrose density gradient sedimentation Tequatrovirus T4
28400
-
 gel filtration Tequatrovirus T4
30690
-
 glycerol density gradient ultracentrifugation analysis Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Tequatrovirus T4 hemimethylated DNA S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA molecular mechanism, effector action of substrates, progressive methylation of extended DNA containing more than one methylation site Tequatrovirus T4
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine hemimethylated DNA Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
S-adenosyl-L-methionine + DNA adenine binding site is the GATC sequence, the adenine is located in the palindromic recognition site GATC, substrate binding mechanism Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Tequatrovirus T4
More enzyme is predominantly a monomer in solution, mechanism of DNA-induced dimerization upon binding to DNA substrate and complex formation, structure and interaction analysis of the enzyme complexed with a 12mer DNA duplex and S-adenosyl-L-homocysteine in a enzyme/duplex ratio of 2, no flipping of adenine out of the DNA duplex Tequatrovirus T4

Synonyms

Synonyms Comment Organism
More enzyme belongs to the Dam MTase family Tequatrovirus T4
T4 Dam DNA methyltransferase
-
 Tequatrovirus T4
T4Dam
-
 Tequatrovirus T4