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Literature summary for 2.1.1.72 extracted from

  • Jois, P.; Madhu, N.; Rao, D.
    Role of histidine residues in EcoP15I DNA methyltransferase activity as probed by chemical modification and site-directed mutagenesis (2008), Biochem. J., 410, 543-553.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
H171A the mutant is almost as active as the wild type enzyme Escherichia coli
H335A the mutant is catalytically inactive and binds to DNA more tightly than the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate the inactivation of the enzyme by diethyl dicarbonate is specific for histidine residues, pre-incubating the methylase with DNA is able to protect the enzyme from diethyl dicarbonate inactivation, hydroxylamine is unable to reverse the effect caused by diethyl dicarbonate Escherichia coli
additional information imidazole (5 mM) and ethanol (0.02% v/v) do not show any effect on enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Escherichia coli EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol ethanol (0.02% v/v) does not show any effect on enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
?
S-adenosyl-L-methionine + pUC19 DNA adenine
-
Escherichia coli S-adenosyl-L-homocysteine + pUC19 DNA 6-methyladenine
-
?

Synonyms

Synonyms Comment Organism
DNA adenine methyltransferase
-
Escherichia coli
EcoP15I MTase
-
Escherichia coli
M.EcoP15I
-
Escherichia coli