Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H171A | the mutant is almost as active as the wild type enzyme | Escherichia coli |
H335A | the mutant is catalytically inactive and binds to DNA more tightly than the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | the inactivation of the enzyme by diethyl dicarbonate is specific for histidine residues, pre-incubating the methylase with DNA is able to protect the enzyme from diethyl dicarbonate inactivation, hydroxylamine is unable to reverse the effect caused by diethyl dicarbonate | Escherichia coli | |
additional information | imidazole (5 mM) and ethanol (0.02% v/v) do not show any effect on enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Escherichia coli | EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | ethanol (0.02% v/v) does not show any effect on enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heparin-Sepharose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine | Escherichia coli | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + pUC19 DNA adenine | - |
Escherichia coli | S-adenosyl-L-homocysteine + pUC19 DNA 6-methyladenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA adenine methyltransferase | - |
Escherichia coli |
EcoP15I MTase | - |
Escherichia coli |
M.EcoP15I | - |
Escherichia coli |