Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.72 extracted from

  • Chernukhin, V.; Seggewiss, J.; Kashirina, Y.; Gonchar, D.; Degtyarev, S.
    Purification and properties of recombinant DNA methyltransferase M2.BstSE of the BstSEI nickase-modification system (2009), Mol. Biol., 43, 8-15.
No PubMed abstract available
Search for more literature for 2.1.1.72

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli RRI cells Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
K+ enzyme activity decreases 100fold in the presence of 100 mM K+ Geobacillus stearothermophilus
Mg2+ enzyme activity decreases in the presence of Mg2+ concentrations above 2.5 mM Geobacillus stearothermophilus
Na+ enzyme activity decreases 100fold in the presence of 100 mM Na+ Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000098
-
 T7 DNA adenine in 33 mM Tris-acetate, pH 7.9, 10 mM magnesium acetate, 66 mM potassium acetate, 1 mM dithiothreitol, at 55°C Geobacillus stearothermophilus
0.0058
-
 S-adenosyl-L-methionine in 33 mM Tris-acetate, pH 7.9, 10 mM magnesium acetate, 66 mM potassium acetate, 1 mM dithiothreitol, at 55°C Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 SDS-PAGE Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine Geobacillus stearothermophilus the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
S-adenosyl-L-methionine + DNA adenine Geobacillus stearothermophilus SE-589 the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
S-adenosyl-L-methionine + T7 DNA adenine Geobacillus stearothermophilus
-
 S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine
-
 ?
S-adenosyl-L-methionine + T7 DNA adenine Geobacillus stearothermophilus SE-589
-
 S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus SE-589
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phosphocellulose column chromatography, hydroxyapatite column chromatography, and Sephacryl S-200 gel filtration Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA adenine the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates Geobacillus stearothermophilus S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
S-adenosyl-L-methionine + DNA adenine the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates Geobacillus stearothermophilus SE-589 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
S-adenosyl-L-methionine + T7 DNA adenine
-
 Geobacillus stearothermophilus S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine
-
 ?
S-adenosyl-L-methionine + T7 DNA adenine
-
 Geobacillus stearothermophilus SE-589 S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine
-
 ?

Synonyms

Synonyms Comment Organism
DNA methyltransferase
-
 Geobacillus stearothermophilus
M2.BstSEI
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.037
-
 T7 DNA adenine in 33 mM Tris-acetate, pH 7.9, 10 mM magnesium acetate, 66 mM potassium acetate, 1 mM dithiothreitol, at 55°C Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Geobacillus stearothermophilus