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Literature summary for 2.1.1.72 extracted from

  • Maier, J.; Albu, R.; Jurkowski, T.; Jeltsch, A.
    Investigation of the C-terminal domain of the bacterial DNA-(adenine N6)-methyltransferase CcrM (2015), Biochimie, 119, 60-67.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D347A the mutant shows wild type-like methylation activity Caulobacter vibrioides
H317A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
H336A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
K318A the mutant shows wild type-like methylation activity Caulobacter vibrioides
N330A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
Q324A the mutant shows wild type-like methylation activity Caulobacter vibrioides
R272A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
R302A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
R350A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides
R354A the mutant shows wild type-like methylation activity Caulobacter vibrioides
S315A the mutant shows a severely decreased methylation activity compared to the wild type enzyme (below 10% activity) Caulobacter vibrioides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
x * 39000, SDS-PAGE Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Caulobacter vibrioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme methylates GANTC sequences but does not specifically bind to DNA containing GANTC sequences Caulobacter vibrioides ?
-
?
S-adenosyl-L-methionine + DNA adenine a biotinylated double stranded 23mer oligodeoxynucleotide with one hemimethylated GANTC site is used as substrate Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Caulobacter vibrioides

Synonyms

Synonyms Comment Organism
CcrM
-
Caulobacter vibrioides
DNA-(adenine N6)-methyltransferase
-
Caulobacter vibrioides