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Literature summary for 2.1.1.77 extracted from

  • David, C.L.; Aswad, D.W.
    Cloning, expression, and purification of rat brain protein L-isoaspartyl methyltransferase (1995), Protein Expr. Purif., 6, 312-318.
    View publication on PubMed

Application

Application Comment Organism
medicine a cloned source of this enzyme should be useful in the identification of cellular substrates and for quantitation and localization of isoaspartyl sites in purified proteins, including recombinant proteins used as pharmaceuticals Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
PCR amplified cDNA cloned into vector pdeltablue and expressed in Escherichia coli Rattus norvegicus

General Stability

General Stability Organism
no stability problems during purification Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00089
-
S-adenosyl-L-methionine native brain enzyme Rattus norvegicus
0.0011
-
S-adenosyl-L-methionine recombinant protein Rattus norvegicus
0.0164
-
isoAspDSIP
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Escherichia coli
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Rattus norvegicus
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate Bos taurus
-
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
bovine
-
Escherichia coli
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
brain
-
Bos taurus
-
testis
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.014
-
-
Rattus norvegicus
0.016 0.02 gamma-globulin as methyl acceptor Rattus norvegicus
0.0168
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, can be stored in 10% glycerol Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + gamma-globulin
-
Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + gamma-globulin
-
Bos taurus S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + isoAspDSIP
-
Rattus norvegicus S-adenosyl-L-homocysteine + ?
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Escherichia coli S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Rattus norvegicus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
-
Bos taurus S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
?

Synonyms

Synonyms Comment Organism
PIMT
-
Rattus norvegicus