Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
mutant enzyme G236E oĆs expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G236E | the mutant has less than 1% of the in vitro activity of the wild type enzyme. The reaction catalyzed by this G236E mutant starts by the methylation of the unsaturated acyl chain at position 10 or 9 yielding a carbocation at position 9 or 10 respectively | Escherichia coli |
G236E | active site mutant, replacing the strictly conserved G236 by a glutamate residue, which corresponds to E146 of the homologous mycolic acid methyltransferase. Mutant has less than 1% of the in vitro activity of the wild-type enzyme and leads to the formation of cyclopropanated fatty acid methyl esters and of new C17 methyl-branched unsaturated fatty acid methyl esters. The double bond of the latters is located at different positions 8, 9 or 10, and the methyl group at position 10 or 9. The reaction catalyzed by G236E mutant thus starts by the methylation of the unsaturated acyl chain at position 10 or 9 yielding a carbocation at position 9 or 10 respectively. It follows then two competing steps, a normal cyclopropanation or hydride shift/elimination events giving different combinations of alkenes | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | Escherichia coli | - |
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P0A9H7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + phospholipid olefinic fatty acid | - |
Escherichia coli | S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CFAS | - |
Escherichia coli |
cyclopropane fatty acid synthase | - |
Escherichia coli |
cyclopropane-fatty-acyl-phospholipid synthase | - |
Escherichia coli |