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Literature summary for 2.1.1.8 extracted from
- Purification and partial characterization of rat kidney histamine-N-methyltransferase (1985), Biochim. Biophys. Acta, 841, 42-49.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | inhibition | Rattus norvegicus |  |
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol, 1 mM, stabilizes | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| histamine | - |
Rattus norvegicus | |
| iodoacetamide | complete inhibition at 0.01 mM | Rattus norvegicus | |
| Methylhistamine | - |
Rattus norvegicus | |
| methylthioadenosine | - |
Rattus norvegicus | |
| p-hydroxymercuribenzoate | complete inhibition at 0.1 mM | Rattus norvegicus | |
| S-adenosylhomocysteine | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0061 | - |
histamine | - |
Rattus norvegicus | |
| 0.0071 | - |
S-adenosyl-L-methionine | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Rattus norvegicus |
| 36000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| renal enzyme | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 10 mM potassium phosphate buffer, pH 7.4, 10% glycerol, 1 mM dithiothreitol, 8 months, 16% loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + histamine | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + Ntau-methylhistamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
90 min, 1 mM dithiothreitol, stable, 40% loss of activity without addition of dithiothreitol | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.4 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 10.3 | about 50% of maximum activity at pH 7.3 and pH 10.3 | Rattus norvegicus |
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