Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-Methoxykaempferol | product inhibition, competitive with respect to 8-hydroxykaempferol | Lotus corniculatus | |
EDTA | in absence of Mg2+ | Lotus corniculatus | |
S-adenosyl-L-homocysteine | product inhibition, non-competitive with respect to S-adenosyl-L-methionine | Lotus corniculatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
8-Hydroxykaempferol | - |
Lotus corniculatus | |
0.053 | - |
S-adenosyl-L-methionine | - |
Lotus corniculatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Lotus corniculatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Lotus corniculatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone | Lotus corniculatus | - |
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone | - |
? | |
S-adenosyl-L-methionine + 8-hydroxykaempferol | Lotus corniculatus | - |
S-adenosyl-L-homocysteine + 8-methoxykaempferol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lotus corniculatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1230fold | Lotus corniculatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone = S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone | mechanism | Lotus corniculatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | - |
Lotus corniculatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lotus corniculatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: glycosides of 8-hydroxyflavonols | Lotus corniculatus | ? | - |
? | |
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone | - |
Lotus corniculatus | S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone | - |
? | |
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone | i.e. 8-hydroxyquercetin | Lotus corniculatus | S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone | i.e. 8-methoxyquercetin | ? | |
S-adenosyl-L-methionine + 8-hydroxykaempferol | - |
Lotus corniculatus | S-adenosyl-L-homocysteine + 8-methoxykaempferol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Lotus corniculatus |