Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | complete inhibition at 2 mM | Asterias rubens | |
EDTA | complete inhibition at 1 mM, reversible by Mn2+ or, to a lesser extent, by Co2+ to 90% and 68% of maximal activity, respectively, no protection by Mg2+ | Asterias rubens | |
Fe3+ | 18% inhibition at 2 mM | Asterias rubens | |
Ni2+ | 84% inhibition at 2 mM | Asterias rubens | |
Zn2+ | 83% inhibition at 2 mM | Asterias rubens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
S-adenosyl-L-methionine | pH 8.0, 25°C, with N-acetylneuraminyl alpha2,3-lactose | Asterias rubens | |
0.044 | - |
N-acetylneuraminyl alpha2,3-lactose | pH 8.0, 25°C | Asterias rubens | |
0.299 | - |
N-acetylneuraminic acid | pH 8.0, 25°C | Asterias rubens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Asterias rubens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at 2 mM | Asterias rubens | |
Co2+ | activates at 2 mM | Asterias rubens | |
Mg2+ | activates at 2 mM | Asterias rubens | |
Mn2+ | activates at 2 mM | Asterias rubens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000 + x * 62000, SDS-PAGE | Asterias rubens |
62000 | - |
x * 58000 + x * 62000, SDS-PAGE | Asterias rubens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + N-acetylneuraminic acid | Asterias rubens | - |
S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Asterias rubens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 22000fold from gonads to homogeneity by detergent-dependent solubilization, ion exchange chromatography, and two cycles of affinity chromatography on an S-adenosyl-L-homocysteine resin | Asterias rubens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gonad | - |
Asterias rubens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71.6 | - |
purified enzyme | Asterias rubens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme preforms specific methylation of endogenous and exogenous glycoconjugate-bound sialic acids, usage of desialized human erythrocyte membranes as substrates, methylation of resialylated erythrocyte membranes, overview | Asterias rubens | ? | - |
? | |
S-adenosyl-L-methionine + CMP-N-acetylneuraminic acid | - |
Asterias rubens | S-adenosyl-L-homocysteine + CMP-N-acetyl-8-O-methyl-neuraminate | - |
? | |
S-adenosyl-L-methionine + colominic acid | - |
Asterias rubens | ? | - |
? | |
S-adenosyl-L-methionine + fetuin | - |
Asterias rubens | ? | - |
? | |
S-adenosyl-L-methionine + N-acetylneuraminic acid | - |
Asterias rubens | S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminate | - |
? | |
S-adenosyl-L-methionine + N-acetylneuraminic acid | free N-acetylneuraminic acid and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred | Asterias rubens | S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminate | NMR product identification | ? | |
S-adenosyl-L-methionine + N-acetylneuraminyl alpha2,3-lactose | free N-acetylneuraminyl alpha2,3-lactose and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred | Asterias rubens | S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminyl alpha2,3-lactose | - |
? | |
S-adenosyl-L-methionine + N-acetylneuraminyl alpha2,6-lactose | free N-acetylneuraminyl alpha2,6-lactose acid and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred | Asterias rubens | S-adenosyl-L-homocysteine + N-acetyl-8-O-methyl-neuraminyl alpha2,6-lactose | - |
? | |
S-adenosyl-L-methionine + N-glycoloylneuraminic acid | free N-glycoloylneuraminic acid and oligosaccharide- or glycoprotein-bound substrate, the latter is preferred | Asterias rubens | S-adenosyl-L-homocysteine + N-glycoloyl-8-O-methyl-neuraminate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000 + x * 62000, SDS-PAGE | Asterias rubens |
Synonyms | Comment | Organism |
---|---|---|
S-adenosyl-L-methionine: sialate-8-O-methyltransferase | - |
Asterias rubens |
sialate-8-O-methyltransferase | - |
Asterias rubens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Asterias rubens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
the purified enzyme is quite unstable at 37°C | Asterias rubens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.3 | broad optimum | Asterias rubens |