Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.B75 extracted from

  • Malla, S.; Koffas, M.A.; Kazlauskas, R.J.; Kim, B.G.
    Production of 7-O-methyl aromadendrin, a medicinally valuable flavonoid, in Escherichia coli (2012), Appl. Environ. Microbiol., 78, 684-694.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.B75

Application

Application Comment Organism
synthesis the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin, which is important in medicinal applications, via sakuranetin from 4-coumaric acid in Escherichia coli, overview Streptomyces avermitilis

Protein Variants

Protein Variants Comment Organism
additional information the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin via sakuranetin from 4-coumaric acid in Escherichia coli, overview. In order to increase the availability of malonyl-CoA, a critical precursor of 7-O-methyl aromadendrin, genes for the acyl-CoA carboxylase alpha- and beta-subunits (nfa9890 and nfa9940), biotin ligase (nfa9950), and acetyl-CoA synthetase (nfa3550) from Nocardia farcinica were also introduced Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q79ZE8 7-O-methyltransferase; gene SaOMT or SAV_2382
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (2S)-naringenin
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
S-adenosyl-L-methionine + (2S)-naringenin
-
 Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
S-adenosyl-L-methionine + dihydrokaempferol
-
 Streptomyces avermitilis S-adenosyl-L-homocysteine + 7-O-methylaromadendrin i.e. 7-O-methyl-dihydrokaempferol ?
S-adenosyl-L-methionine + dihydrokaempferol
-
 Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 7-O-methylaromadendrin i.e. 7-O-methyl-dihydrokaempferol ?

Synonyms

Synonyms Comment Organism
7-O-methyltransferase
-
 Streptomyces avermitilis
SaOMT
-
 Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Streptomyces avermitilis

General Information

General Information Comment Organism
metabolism 7-O-methyl aromadendrin biosynthesis pathway from initial 4-coumaric acid, overview Streptomyces avermitilis