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Literature summary for 2.1.1.B85 extracted from
- Three new O-methyltransferases are sufficient for all O-methylation reactions of ipecac alkaloid biosynthesis in root culture of Psychotria ipecacuanha (2010), J. Biol. Chem., 285, 7722-7738.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Carapichea ipecacuanha |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Carapichea ipecacuanha | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38700 | - |
x * 38700, calculated from sequence | Carapichea ipecacuanha |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + cephaeline | Carapichea ipecacuanha | the enzyme is involved in emetine biosynthesis. IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline | S-adenosyl-L-homocysteine + emetine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carapichea ipecacuanha | D3KY99 | i.e. Psychotria ipecacuanha | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| N-terminal His-tagged IpeOMT2 enzyme | Carapichea ipecacuanha |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Carapichea ipecacuanha | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline. IpeOMT2 shows broad substrate specificity towards the intermediates in emetine biosynthesis. The regional substrate specificity for 7'-O-demethylcephaeline (the penultimate step of emetine biosynthesis) is restricted to 7'-O-methylation | Carapichea ipecacuanha | ? | - |
? | |
| S-adenosyl-L-methionine + 7'-O-demethylcephaeline | - |
Carapichea ipecacuanha | S-adenosyl-L-homocysteine + cephaeline | - |
? | |
| S-adenosyl-L-methionine + cephaeline | the enzyme is involved in emetine biosynthesis. IpeOMT2 does not catalyze the 6'-O-methylation of cephaeline | Carapichea ipecacuanha | S-adenosyl-L-homocysteine + emetine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38700, calculated from sequence | Carapichea ipecacuanha |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IpeOMT2 | - |
Carapichea ipecacuanha |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Carapichea ipecacuanha |
| 45 | - |
- |
Carapichea ipecacuanha |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Carapichea ipecacuanha |
| 7.5 | - |
assay at | Carapichea ipecacuanha |
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Release 2023.1 (January 2023)
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