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Literature summary for 2.1.2.1 extracted from

  • Fujioka, M.
    Purification and properties of serine hydroxymethylase from soluble and mitochondrial fractions of rabbit liver (1969), Biochim. Biophys. Acta, 185, 338-349.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mitochondrial enzyme Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Oryctolagus cuniculus
DL-2-methylserine
-
Oryctolagus cuniculus
EDTA weak Oryctolagus cuniculus
hydrazine
-
Oryctolagus cuniculus
additional information not: iodoacetate; not: NaN3, mono- or divalent cations, 2-mercaptoethanol, DTT; not: purine nucleoside mono-, di- and triphosphates Oryctolagus cuniculus
N-ethylmaleimide
-
Oryctolagus cuniculus
NH2OH weak Oryctolagus cuniculus
PCMB
-
Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
L-serine mitochondrial enzyme Oryctolagus cuniculus
1.3
-
L-serine cytosolic enzyme Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Oryctolagus cuniculus 5737
-
mitochondrion
-
Oryctolagus cuniculus 5739
-
additional information isozymes in cytosol and mitochondria Oryctolagus cuniculus
-
-
additional information immunochemically different Oryctolagus cuniculus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not Oryctolagus cuniculus
Mg2+ not Oryctolagus cuniculus
Mn2+ not Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
mitochondria, sedimentation equilibrium centrifugation Oryctolagus cuniculus
185000
-
soluble, high speed sedimentation equilibrium centrifugation Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Oryctolagus cuniculus
10
-
soluble Oryctolagus cuniculus
15.5
-
mitochondria Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
5°C, precipitation after several days Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-methylserine + tetrahydrofolate at high concentrations of enzyme Oryctolagus cuniculus D-alanine + 5,10-methylenetetrahydrofolate
-
?
L-serine + tetrahydrofolate
-
Oryctolagus cuniculus glycine + 5,10-methylenetetrahydrofolate + H2O
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 cytosol: about 60% of maximal activity at pH 6, about 70% at 8.5, mitochondria: about 40% of maximal activity at pH 6.0 and 8.5 Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement, 4 mol per mol enzyme Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.9
-
DL-2-methylserine soluble fraction Oryctolagus cuniculus
11.2
-
DL-2-methylserine mitochondrial enzyme Oryctolagus cuniculus