Crystallization (Comment) | Organism |
---|---|
mitochondrial enzyme | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Oryctolagus cuniculus | |
DL-2-methylserine | - |
Oryctolagus cuniculus | |
EDTA | weak | Oryctolagus cuniculus | |
hydrazine | - |
Oryctolagus cuniculus | |
additional information | not: iodoacetate; not: NaN3, mono- or divalent cations, 2-mercaptoethanol, DTT; not: purine nucleoside mono-, di- and triphosphates | Oryctolagus cuniculus | |
N-ethylmaleimide | - |
Oryctolagus cuniculus | |
NH2OH | weak | Oryctolagus cuniculus | |
PCMB | - |
Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-serine | mitochondrial enzyme | Oryctolagus cuniculus | |
1.3 | - |
L-serine | cytosolic enzyme | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Oryctolagus cuniculus | 5737 | - |
mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
additional information | isozymes in cytosol and mitochondria | Oryctolagus cuniculus | - |
- |
additional information | immunochemically different | Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not | Oryctolagus cuniculus | |
Mg2+ | not | Oryctolagus cuniculus | |
Mn2+ | not | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
mitochondria, sedimentation equilibrium centrifugation | Oryctolagus cuniculus |
185000 | - |
soluble, high speed sedimentation equilibrium centrifugation | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Oryctolagus cuniculus |
10 | - |
soluble | Oryctolagus cuniculus |
15.5 | - |
mitochondria | Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
5°C, precipitation after several days | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-methylserine + tetrahydrofolate | at high concentrations of enzyme | Oryctolagus cuniculus | D-alanine + 5,10-methylenetetrahydrofolate | - |
? | |
L-serine + tetrahydrofolate | - |
Oryctolagus cuniculus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | cytosol: about 60% of maximal activity at pH 6, about 70% at 8.5, mitochondria: about 40% of maximal activity at pH 6.0 and 8.5 | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement, 4 mol per mol enzyme | Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
DL-2-methylserine | soluble fraction | Oryctolagus cuniculus | |
11.2 | - |
DL-2-methylserine | mitochondrial enzyme | Oryctolagus cuniculus |