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Literature summary for 2.1.2.1 extracted from

  • Ramesh, K.S.; Rao, N.A.
    Purification and physicochemical, kinetic and immunological properties of allosteric serine hydroxymethyltransferase from monkey liver (1980), Biochem. J., 187, 623-636.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information in the presence of 1mM dithiothreitol + 1mM pyridoxal 5-phosphate + 20 mM EDTA: 29fold increase in the specific activity Macaca radiata

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Macaca radiata
DTT stabilizes Macaca radiata
EDTA stabilizes Macaca radiata
pyridoxal 5'-phosphate stabilizes Macaca radiata

Inhibitors

Inhibitors Comment Organism Structure
aminopterin
-
Macaca radiata
D-cycloserine
-
Macaca radiata
Dichloromethotrexate
-
Macaca radiata
dihydrofolate
-
Macaca radiata
glycine
-
Macaca radiata
methotrexate
-
Macaca radiata
additional information inhibition kinetics Macaca radiata
tetrahydrofolate derivatives
-
Macaca radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information allosteric kinetics Macaca radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm 75% of the enzyme activity Macaca radiata 5737
-
microsome 3% of the enzyme activity Macaca radiata
-
-
mitochondrion 5% of the enzyme activity Macaca radiata 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
4 * 52000, SDS-PAGE Macaca radiata
208000
-
gel filtration Macaca radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + tetrahydrofolate Macaca radiata regulatory protein glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Macaca radiata
-
Bonnet monkey
-

Purification (Commentary)

Purification (Comment) Organism
heat-denaturation omitted Macaca radiata

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Macaca radiata

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Macaca radiata
-
kidney
-
Macaca radiata
-
additional information distribution Macaca radiata
-
pancreas
-
Macaca radiata
-
skeletal muscle
-
Macaca radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Macaca radiata
3.4
-
-
Macaca radiata

Storage Stability

Storage Stability Organism
-20°C, 6-8 months in the presence of pyridoxal 5'-phosphate, 2-mercaptoethanol, DTT and EDTA Macaca radiata
0°C, at least 2 months in the presence of pyridoxal 5'-phosphate, 2-mercaptoethanol, DTT and EDTA Macaca radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
Macaca radiata glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
L-serine + tetrahydrofolate regulatory protein Macaca radiata glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
additional information insoluble enzyme/antibody-complex shows 90% of original activity Macaca radiata ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 52000, SDS-PAGE Macaca radiata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Macaca radiata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
L-serine protects against thermal inactivation Macaca radiata
additional information
-
enzyme-antibody complex more stable to elevated temperatures than free enzyme, allosteric effectors fail to protect free enzyme Macaca radiata
50
-
20 min stable Macaca radiata
65
-
85% loss of activity after 20 min Macaca radiata
70
-
complete loss of activity after 6 min, enzyme-antibody complex: 30% loss of activity after 20 min Macaca radiata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Macaca radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 4.2 Macaca radiata
7.4
-
-
Macaca radiata

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement, 4 mol per mol enzyme Macaca radiata
tetrahydrofolate
-
Macaca radiata