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Literature summary for 2.1.2.1 extracted from

  • Strong, W.B.; Tendler, S.J.; Seither, R.L.; Goldman, I.D.; Schirch, V.
    Purification and properties of serine hydroxymethyltransferase and C1-tetrahydrofolate synthase from L1210 cells (1990), J. Biol. Chem., 265, 12149-12155.
    View publication on PubMed
Search for more literature for 2.1.2.1

Inhibitors

Inhibitors Comment Organism Structure
L-serine
-
 Mus musculus
tetrahydrofolate derivatives
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mus musculus
0.15 0.9 L-serine
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Mus musculus 5737
-
mitochondrion
-
 Mus musculus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, SDS-PAGE Mus musculus
215000
-
 HPLC gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + tetrahydrofolate Mus musculus main glycine source for purine biosynthetic pathway in ureide biogenesis glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate Mus musculus key enzyme of serine pathway for assimilation of C1-compounds glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Mus musculus
a

Source Tissue

Source Tissue Comment Organism Textmining
L-1210 cell transformed cells Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.7
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
 Mus musculus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate main glycine source for purine biosynthetic pathway in ureide biogenesis Mus musculus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate key enzyme of serine pathway for assimilation of C1-compounds Mus musculus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 56000, SDS-PAGE Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information
-
 Mus musculus