General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Vigna radiata |
dialysis inactivates | Vigna radiata |
EDTA stabilizes | Vigna radiata |
KCl, NaCl or LiCl: denaturation, folate protects | Vigna radiata |
L-serine stabilizes | Vigna radiata |
tetrahydrofolate stabilizes | Vigna radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminopterin | - |
Vigna radiata | |
D-cycloserine | - |
Vigna radiata | |
Dichloromethotrexate | - |
Vigna radiata | |
DL-2-methylserine | - |
Vigna radiata | |
DL-O-Methylserine | - |
Vigna radiata | |
glycine | - |
Vigna radiata | |
methotrexate | - |
Vigna radiata | |
O-phosphoserine | - |
Vigna radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Vigna radiata | |
1.8 | - |
L-serine | - |
Vigna radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Vigna radiata | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Vigna radiata |
205000 | - |
gel filtration | Vigna radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Vigna radiata | regulatory protein | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | Vigna radiata | key enzyme of serine pathway for assimilation of C1-compounds | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata | - |
mung bean | - |
Purification (Comment) | Organism |
---|---|
- |
Vigna radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | germinating | Vigna radiata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Vigna radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Vigna radiata | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
L-serine + tetrahydrofolate | regulatory protein | Vigna radiata | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
L-serine + tetrahydrofolate | key enzyme of serine pathway for assimilation of C1-compounds | Vigna radiata | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Vigna radiata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
2 optima: 35°C and 55°C | Vigna radiata |
55 | - |
2 optima: 35°C and 55°C | Vigna radiata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 75 | about half-maximal activity at 25°C and 75°C | Vigna radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Vigna radiata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | about half-maximal activity at pH 6.5 and 9.5 | Vigna radiata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | not essential | Vigna radiata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.02 | - |
D-cycloserine | - |
Vigna radiata | |
2.3 | - |
glycine | - |
Vigna radiata |