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Literature summary for 2.1.2.1 extracted from

  • Nakamura, K.D.; Trewyn, R.W.; Parks, L.W.
    Purification and characterization of serine transhydroxy-methylase from Saccharomyces cerevisiae (1973), Biochim. Biophys. Acta, 327, 328-335.
    View publication on PubMed
Search for more literature for 2.1.2.1

General Stability

General Stability Organism
DTT stabilizes Saccharomyces cerevisiae
pyridoxal 5'-phosphate stabilizes Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information not: EDTA; not: purine nucleoside mono-, di- and triphosphates Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae
0.15 0.9 L-serine
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation at 0.5-10 mM Saccharomyces cerevisiae
Mg2+ activation at 0.5-10 mM Saccharomyces cerevisiae
Mn2+ activation at 0.5-10 mM Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000 200000 gel filtration, sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + tetrahydrofolate Saccharomyces cerevisiae key enzyme of serine pathway for assimilation of C1-compounds glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate Saccharomyces cerevisiae 3701B key enzyme of serine pathway for assimilation of C1-compounds glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae 3701B
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-10°C, 50% loss of activity in 10 h Saccharomyces cerevisiae
4°C, 50% loss of activity in 10 h Saccharomyces cerevisiae
frozen, up to a month in the presence of 30% v/v glycerol Saccharomyces cerevisiae
glycerol, 30% v/v, enhances thermal stability Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
 Saccharomyces cerevisiae glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate key enzyme of serine pathway for assimilation of C1-compounds Saccharomyces cerevisiae glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate
-
 Saccharomyces cerevisiae 3701B glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
L-serine + tetrahydrofolate key enzyme of serine pathway for assimilation of C1-compounds Saccharomyces cerevisiae 3701B glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 glycerol, 30% v/v, enhances thermal stability Saccharomyces cerevisiae
55
-
 t1/2: about 2 min, glycerol stabilizes Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9.5
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae
tetrahydrofolate requirement with L-serine or L-2-methylserine as substrate Saccharomyces cerevisiae