Literature summary for 2.1.2.1 extracted from

Rao, J.V.K.; Prakash, V.; Appaji Rao, N.; Savithri, H.S.
The role of Glu74 and Tyr82 in the reaction catalyzed by sheep liver cytosolic serine hydroxymethyltransferase (2000), Eur. J. Biochem., 267, 5967-5976.

Search for more literature for 2.1.2.1

Application

Application	Comment	Organism
medicine	enzyme is a potential target for cancer chemotherapy	Ovis aries

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Ovis aries

Protein Variants

Protein Variants	Comment	Organism
E74K	specific activities drastically reduced with serine as substrate, but D-alanine transamination and allothreonine cleavage at rates comparable with wild-type enzyme	Ovis aries
E74Q	specific activities drastically reduced with serine as substrate, but D-alanine transamination and allothreonine cleavage at rates comparable with wild type enzyme	Ovis aries
Y82F	95% loss of activity, kcat and Km decreased, a role in stabilizing the quinonoid intermediate	Ovis aries

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.15	0.9	L-serine	mutant enzyme Y82F	Ovis aries
1	-	L-serine	-	Ovis aries
1.5	-	L-serine	mutant enzyme E74Q	Ovis aries

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53000	-	4 * 53000, mutant enzyme	Ovis aries
220000	-	mutant enzyme	Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-serine + tetrahydrofolate	Ovis aries	-	glycine + 5,10-methylenetetrahydrofolate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Ovis aries	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine	mechanism	Ovis aries	a

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.012	-	mutant enzyme E74Q	Ovis aries
0.16	-	mutant enzyme Y82F	Ovis aries
4.2	-	-	Ovis aries

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine + tetrahydrofolate	-	Ovis aries	glycine + 5,10-methylenetetrahydrofolate + H2O	-	?
additional information	enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids	Ovis aries	?	-	?

Subunits

Subunits	Comment	Organism
dimer	removal of the bound pyridoxal 5'-phosphate from the mutant tetrameric enzyme leads to dissociation to a dimer	Ovis aries
homotetramer	4 * 53000, mutant enzyme	Ovis aries

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
additional information	-	thermograms of enzyme and mutant enzyme in the absence and presence of L-serine	Ovis aries

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	Tm: 55°C, increased to 65°C in the presence of L-serine, Tm: 68°C mutant enzyme Y82F in the presence of serine	Ovis aries
additional information	-	L-serine increases the thermal stability	Ovis aries

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.01	-	L-serine	mutant enzyme E74Q	Ovis aries
0.0967	-	L-serine	mutant enzyme Y82F	Ovis aries
4.1	-	L-serine	-	Ovis aries

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Ovis aries

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Release 2023.1 (January 2023)