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Literature summary for 2.1.2.1 extracted from

  • Schjoerring, J.K.; Maeck, G.; Nielsen, K.H.; Husted, S.; Suzuki, A.; Driscoll, S.; Boldt, R.; Bauwe, H.
    Antisense reduction of serine hydroxymethyltransferase results in diurnal displacement of NH4+ assimilation in leaves of Solanum tuberosum (2006), Plant J., 45, 71-82.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
full-length cDNA clone SST18 encoding potato SHMT inserted in antisense orientation into the pBIN19 based vector L700 and introduced into the genome of Solanum tuberosum Solanum tuberosum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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Solanum tuberosum 5739
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000 200000
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Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
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cv. Solara, antisense lines G310-10, G310-16, G310-76, G310-101 and G310-106
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Source Tissue

Source Tissue Comment Organism Textmining
leaf SHMT activity of antisense lines is 70-90% lower than in the wild-type, negative effects of antisensed SHMT becomes increasingly severe with plant age Solanum tuberosum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate SHMT antisense plants display lower photosynthetic capacity and accumulate glycine in light, glycine is converted to serine in the second half of the light period, serine shows an inverse diurnal rhythm and reaches highest values in darkness, glycine/serine conversion is independent of light in the transformant, but not in the wild-type Solanum tuberosum glycine + 5,10-methylenetetrahydrofolate + H2O
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Synonyms

Synonyms Comment Organism
serine hydroxymethyl transferase
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Solanum tuberosum
SHMT
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Solanum tuberosum