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Literature summary for 2.1.2.1 extracted from

  • Sopitthummakhun, K.; Maenpuen, S.; Yuthavong, Y.; Leartsakulpanich, U.; Chaiyen, P.
    Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues (2009), FEBS J., 276, 4023-4036.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plasmodium vivax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
tetrahydrofolate in 50 mM HEPES buffer (pH 7.0), at 25°C Plasmodium vivax
0.18
-
L-serine in 50 mM HEPES buffer (pH 7.0), at 25°C Plasmodium vivax
47
-
D-serine apparent value, in 50 mM HEPES buffer (pH 7.0), at 25°C Plasmodium vivax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, SDS-PAGE Plasmodium vivax
72400
-
gel filtration Plasmodium vivax

Organism

Organism UniProt Comment Textmining
Plasmodium vivax
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-25 gel filtration Plasmodium vivax

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
crude extract, in 50 mM HEPES (pH 7.0), 1 mM dithiothreitol and 0.5 mM EDTA at 25°C Plasmodium vivax
1.86
-
after 3.8fold purification, in 50 mM HEPES (pH 7.0), 1 mM dithiothreitol and 0.5 mM EDTA at 25°C Plasmodium vivax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-serine + tetrahydrofolate the binding affinity for D-serine is 150fold lower than that of L-serine Plasmodium vivax glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
L-serine + tetrahydrofolate L-serine is the physiological substrate Plasmodium vivax glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
additional information SHMT also catalyzes the tetrahydrofolate-independent retro-aldol cleavage of 3-hydroxy amino acids Plasmodium vivax ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 49000, SDS-PAGE Plasmodium vivax

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Plasmodium vivax
SHMT
-
Plasmodium vivax

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 30 the catalytic activity increases upon temperature increment Plasmodium vivax

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
D-serine in 50 mM HEPES buffer (pH 7.0), at 25°C Plasmodium vivax
0.98
-
L-serine in 50 mM HEPES buffer (pH 7.0), at 25°C Plasmodium vivax
0.98
-
tetrahydrofolate in 50 mM HEPES buffer (pH 7.0), at 25°C Plasmodium vivax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
in 50 mM HEPES Plasmodium vivax

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10
-
Plasmodium vivax

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate the Kd for binding of the enzyme and pyridoxal 5'-phosphate is 0.00014 mM Plasmodium vivax