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Literature summary for 2.1.2.1 extracted from

  • Huang, J.; Chen, L.; Hu, N.; Jiang, W.; Wu, G.; Liu, Z.
    Characterization of a novel serine hydroxymethyltransferase isolated from marine bacterium Arthrobacter sp. and its application on L-serine production (2015), Ann. Microbiol., 65, 1689-1698.
    View publication on PubMed
Search for more literature for 2.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
gene glyA, DNA and amino acid sequence determination and analysis, thermal asymmetric interlaced PCR, TAIL-PCR, sequence comparisons and phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ strong inhibition at 1 mM Arthrobacter sp.
CTAB
-
 Arthrobacter sp.
Cu2+ strong inhibition at 1 mM Arthrobacter sp.
Fe2+ strong inhibition at 1 mM Arthrobacter sp.
Hg2+ strong inhibition at 1 mM Arthrobacter sp.
Mn2+ strong inhibition at 1 mM Arthrobacter sp.
SDS
-
 Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates slightly Arthrobacter sp.
EDTA activates slightly Arthrobacter sp.
Mg2+ activates slightly Arthrobacter sp.
additional information K+, Na+ and NH4+ show no appreciable impact on the SHMT activity Arthrobacter sp.
Pb2+ activates slightly Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O Arthrobacter sp.
-
 tetrahydrofolate + L-serine
-
 r
5,10-methylenetetrahydrofolate + glycine + H2O Arthrobacter sp. MCCC 1A05493
-
 tetrahydrofolate + L-serine
-
 r
tetrahydrofolate + L-serine Arthrobacter sp.
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
tetrahydrofolate + L-serine Arthrobacter sp. MCCC 1A05493
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 gene glyA
-
Arthrobacter sp. MCCC 1A05493
-
 gene glyA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Arthrobacter sp. tetrahydrofolate + L-serine
-
 r
5,10-methylenetetrahydrofolate + glycine + H2O
-
 Arthrobacter sp. MCCC 1A05493 tetrahydrofolate + L-serine
-
 r
tetrahydrofolate + L-serine
-
 Arthrobacter sp. 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r
tetrahydrofolate + L-serine
-
 Arthrobacter sp. MCCC 1A05493 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
 Arthrobacter sp.
SHMT
-
 Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Arthrobacter sp.

General Information

General Information Comment Organism
evolution the enzyme belongs to the alpha-family of fold type I, and pyridoxal 5'-phosphate-dependent enzymes Arthrobacter sp.
physiological function SHMT plays an important role in the assimilation of C1 compounds, yielding the main L-serine intermediate Arthrobacter sp.