Application | Comment | Organism |
---|---|---|
medicine | glycinamide ribonucleotide transformylase is a validated target for cancer chemotherapy | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the pET17b vector and into the pMC8HdeltaT vector for expression in Escherichia coli BL21Star(DE3)pRARE cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D144E | catalytically inactive mutant | Homo sapiens |
D144H | catalytically inactive mutant | Homo sapiens |
H108K | catalytically inactive mutant | Homo sapiens |
H108R | catalytically inactive mutant | Homo sapiens |
K170R | catalytically active mutant | Homo sapiens |
N106D | catalytically inactive mutant | Homo sapiens |
N106H | catalytically inactive mutant | Homo sapiens |
N106Q | catalytically active mutant | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
10-formyl-5,8-dideazafolate | wild type enzyme | Homo sapiens | |
0.0011 | - |
beta-glycinamide ribonucleotide | wild type enzyme | Homo sapiens | |
0.0015 | - |
10-formyl-5,8-dideazafolate | mutant K170R | Homo sapiens | |
0.0018 | - |
beta-glycinamide ribonucleotide | mutant K170R | Homo sapiens | |
0.0196 | - |
10-formyl-5,8-dideazafolate | mutant N106Q | Homo sapiens | |
0.0295 | - |
beta-glycinamide ribonucleotide | mutant N106Q | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
108000 | - |
human GART comprises the C-terminal domain of a 108 kDa, trifunctional enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a NiHisTrap HP column, the affinity tag is removed by cleavage with TEV protease | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyl-5,8-dideazafolate + N1-(5-phospho-D-ribosyl)glycinamide | - |
Homo sapiens | ? | - |
? | |
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide | - |
Homo sapiens | tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide | - |
? | |
beta-glycinamide ribonucleotide + 10-formyltetrahydrofolate | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GART | - |
Homo sapiens |
glycinamide ribonucleotide transformylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.5 | - |
beta-glycinamide ribonucleotide | wild type enzyme | Homo sapiens | |
7.7 | - |
beta-glycinamide ribonucleotide | mutant K170R | Homo sapiens | |
14 | - |
beta-glycinamide ribonucleotide | mutant N106Q | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
folate | - |
Homo sapiens |