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Literature summary for 2.1.2.3 extracted from

  • Wolan, D.W.; Greasley, S.E.; Beardsley, G.P.; Wilson, I.A.
    Structural insights into the avian AICAR transformylase mechanism (2002), Biochemistry, 41, 15505-15513.
    View publication on PubMed

Application

Application Comment Organism
medicine
-
Gallus sp.
medicine target for development of inhibitors with potential use as chemotherapeutic agents Homo sapiens
medicine potential target for development of antineoplastic drugs Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
ATICcDNA cloned into a pET28a vector and transformed into Escherichia coli BL21.DE3 for overexpression Gallus sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
ATIC complex crystallized in monoclinic space group P2(1), a = 56.48 A, b = 107.88 A, c = 103.86 A and beta = 91.2° Gallus sp.

Inhibitors

Inhibitors Comment Organism Structure
4-amino-10-methylpteroylglutamic acid methotrexate, non-competitive inhibitor Gallus sp.
4-amino-10-methylpteroylglutamic acid methotrexate, non-competitive inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0168
-
5-aminoimidazole-4-carboxamide ribonucleotide
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Homo sapiens penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide Gallus sp. penultimate and final steps in the de novo purine biosynthesis pathway tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r

Organism

Organism UniProt Comment Textmining
Gallus sp.
-
avian
-
Homo sapiens
-
human
-

Purification (Commentary)

Purification (Comment) Organism
-
Gallus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Homo sapiens tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamide penultimate and final steps in the de novo purine biosynthesis pathway Gallus sp. tetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
-
r
additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Homo sapiens ?
-
?
additional information in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene Gallus sp. ?
-
?
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Homo sapiens tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
-
Gallus sp. tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
-
r

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens
dimer
-
Gallus sp.

Cofactor

Cofactor Comment Organism Structure
N10-formyltetrahydrofolate
-
Homo sapiens
N10-formyltetrahydrofolate
-
Gallus sp.