Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aminobacter sp. AJ110403 |
expression in Escherichia coli | Ensifer sp. AJ110404 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
tetrahydrofolate | - |
Aminobacter sp. AJ110403 | |
0.228 | - |
tetrahydrofolate | - |
Ensifer sp. AJ110404 | |
1.5 | - |
2-methyl-L-serine | - |
Aminobacter sp. AJ110403 | |
1.88 | - |
2-methyl-L-serine | - |
Ensifer sp. AJ110404 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
gel filtration | Aminobacter sp. AJ110403 |
80000 | - |
gel filtration | Ensifer sp. AJ110404 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aminobacter sp. AJ110403 | B2DEU8 | - |
- |
Ensifer sp. AJ110404 | B2DEV1 | - |
- |
Purification (Comment) | Organism |
---|---|
column chromatography, 16.4% yield | Ensifer sp. AJ110404 |
column chromatography, 72.8% yield | Aminobacter sp. AJ110403 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1.25 U/mg, cell-free extract | Ensifer sp. AJ110404 |
additional information | - |
4.75 U/mg, cell-free extract | Aminobacter sp. AJ110403 |
additional information | - |
7.10 U/mg, after purification | Ensifer sp. AJ110404 |
additional information | - |
8.32 U/mg, after purification | Aminobacter sp. AJ110403 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-L-serine + tetrahydrofolate | assay at pH 7.4, 30°C | Aminobacter sp. AJ110403 | D-alanine + 5,10-methylenetetrahydrofolate | - |
r | |
2-methyl-L-serine + tetrahydrofolate | assay at pH 7.4, 30°C | Ensifer sp. AJ110404 | D-alanine + 5,10-methylenetetrahydrofolate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Aminobacter sp. AJ110403 |
homodimer | - |
Ensifer sp. AJ110404 |
Synonyms | Comment | Organism |
---|---|---|
alpha-methylserine hydroxymethyltransferase | - |
Aminobacter sp. AJ110403 |
alpha-methylserine hydroxymethyltransferase | - |
Ensifer sp. AJ110404 |
MSHMT | - |
Aminobacter sp. AJ110403 |
MSHMT | - |
Ensifer sp. AJ110404 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aminobacter sp. AJ110403 |
30 | - |
assay at | Ensifer sp. AJ110404 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
value about | Aminobacter sp. AJ110403 |
50 | - |
value about | Ensifer sp. AJ110404 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Aminobacter sp. AJ110403 |
7.4 | - |
assay at | Ensifer sp. AJ110404 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Aminobacter sp. AJ110403 |
6 | 7.5 | - |
Ensifer sp. AJ110404 |