Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 | Tequatrovirus T4 |
structural gene 42 encodes enzyme | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
E60D | mutant with decreased enzyme activity, impaired enzyme function in vitro and in vivo, uncoupling of early steps from later steps in catalysis | Tequatrovirus T4 |
E60Q | mutant with decreased enzyme activity, impaired enzyme function in vitro and in vivo, uncoupling of early steps from later steps in catalysis | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-deazatetrahydrofolate | 1 mM, 54% inhibition | Tequatrovirus T4 | |
5-fluorodeoxyuridylate | mechanism-based inhibitor | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28487 | - |
2 * 28487 | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | Tequatrovirus T4 | - |
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | involved in biosynthesis of DNA precursor | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
Escherichia coli BL21 infected with bacteriophage T4 | - |
Purification (Comment) | Organism |
---|---|
purification of wild-type enzyme and mutants E60Q and E60D | Tequatrovirus T4 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | ordered bi-bi mechanism | Tequatrovirus T4 | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | catalytic mechanism | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | - |
Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | involved in biosynthesis of DNA precursor | ? | |
5,10-methylenetetrahydrofolate + H2O + deoxycytidylate | role of active site Glu-60 is to accelerate bond cleavage between N5 of tetrahydrofolate and the methylene being transferred | Tequatrovirus T4 | tetrahydrofolate + 5-hydroxymethyldeoxycytidylate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 28487 | Tequatrovirus T4 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
deoxycytidylate | mutant E60Q | Tequatrovirus T4 | |
0.007 | - |
deoxycytidylate | mutant E60D | Tequatrovirus T4 | |
12.7 | - |
deoxycytidylate | wild-type enzyme | Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Tequatrovirus T4 | |
tetrahydrofolate | - |
Tequatrovirus T4 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
5-deazatetrahydrofolate | - |
Tequatrovirus T4 |