Cloned (Comment) | Organism |
---|---|
1.3S subunit cloned and expressed in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
Protein Variants | Comment | Organism |
---|---|---|
M88L | partial reaction 1: 99% loss activity, partial reaction 2: 65% loss of activity, possibly alterations in the microenvironment of the biocytin | Propionibacterium freudenreichii subsp. shermanii |
M90L | partial reaction 1: 50% loss of activity, partial reaction 2: 115% activity compared to the 1.3 wild-type enzyme | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate | partial reaction 1 is catalysed specifically by the 12S subunit, partial reaction 2 is catalysed specifically by the 5S subunit | Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionyl-CoA + oxaloacetate | two partial reactions | Propionibacterium freudenreichii subsp. shermanii | (S)-methylmalonyl-CoA + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | the 26S holoenzyme consists of a hexameric central 12S subunit, 360000 Da and six peripheral dimeric 5S subunits, 6 * 120000 Da each of which is linked to the central subunit by 2 biotinyl 1.3S subunits, 12 * MW 12000 | Propionibacterium freudenreichii subsp. shermanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | requirement | Propionibacterium freudenreichii subsp. shermanii | |
biotin | covalently linked to the 1.3 subunit to the epsilon-amino group of Lys-89, which lies in the conserved sequence Ala-Met-Lys-Met | Propionibacterium freudenreichii subsp. shermanii |