Any feedback?
Literature summary for 2.1.3.12 extracted from
- Characterization of NovP and NovN: completion of novobiocin biosynthesis by sequential tailoring of the noviosyl ring (2004), Angew. Chem. Int. Ed. Engl., 43, 67-70.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces niveus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
decarbamoylnovobiocin | pH 8.5, 22°C | Streptomyces niveus |  |
| 0.0051 | - |
Carbamoyl phosphate | pH 8.5, 22°C | Streptomyces niveus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + decarbamoylnovobiocin | Streptomyces niveus | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | phosphate + novobiocin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces niveus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| N-His6-tagged protein | Streptomyces niveus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + decarbamoylnovobiocin | - |
Streptomyces niveus | phosphate + novobiocin | - |
? | |
| carbamoyl phosphate + decarbamoylnovobiocin | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | Streptomyces niveus | phosphate + novobiocin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NovN | - |
Streptomyces niveus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Streptomyces niveus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.1 | - |
decarbamoylnovobiocin | pH 8.5, 22°C | Streptomyces niveus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Streptomyces niveus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | activation, carbamoyltransferase activity of NovN is dependent upon Mg-ATP | Streptomyces niveus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in the biosynthesis of the aminocoumarin antibiotics, novobiocin | Streptomyces niveus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 891 | - |
decarbamoylnovobiocin | pH 8.5, 22°C | Streptomyces niveus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
